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Agilent Genomics

XL2-Blue MRF’ Ultracompetent Cells

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Ultracompetent cells for high efficiency cloning of methylated DNA

Ultracompetent cells for the highest efficiency cloning of a variety of plasmids

Deficient in all known E. coli K12 restriction systems to eliminate cleavage of eukaryotic DNA with methylation patterns that are different than the E. coli host methylation patterns.

Cloning methylated DNA is more efficient with restriction-minus competent cells

When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by theE. coli host restriction systems. Cleavage of DNA before host replication creates libraries that lack complete representation. Our MR (Minus Restriction) series of competent cells are deficient in all known E. coli K12 restriction systems and make it possible to clone methylated DNA, which can be very useful in epigenetic studies where DNA methylation, instead of changes in DNA sequence, may be associated with heritable changes in gene expression or cellular phenotype.

XL2-Blue MRF´ ultracompetent cells are high-efficiency derivatives of our XL1-Blue MRF´ supercompetent cells. Using ultracompetent cell technology, we have achieved transformation efficiencies of greater than 5 × 10^9 transformants/µg of pUC18 DNA, making these ultracompetent cells an ideal choice for high-efficiency cloning. The XL2-Blue MRF´ (Minus Restriction) strain is deficient in all known restriction systems (McrA–, McrCB–, McrF–, Mrr–, HsdR–), preventing cleavage of cloned DNA that carries cytosine and/or adenine methylation, which is often present in eukaryotic DNA and cDNA. XL2-Blue MRF´ cells are restriction minus. The strain is endonuclease deficient (endA), greatly improving the quality of miniprep DNA, and recombination deficient (recA), helping to ensure insert stability. These cells allow blue-white screening for recombinant plasmids.

This product is only available to U.S. Domestic Customers.

For research use only, not to be used in diagnostic procedures.

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Product Detail
Thomas No.
CHM01R306
Mfr. No.
200150
UN #
UN1845
Description
XL2-Blue Ultracompetent Cells 10 x 0.1 mL, Highest cloning efficiency, 5 x 10<sup>9</sup> transformants/µg

Domestic Only (Domestic Only)

Packaging Size
10 x 0.1 mL
list price/quantitytotal
$0.00
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Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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