Cetyltrimethylammonium bromide is a phase transfer catalyst ideal for the precipitation of DNA. Hexadecyltrimethylammonium bromide is a bactericidal, cationic detergent. Its activity is neutralized by soaps and anionic detergents (i.e., SDS). Trimethylammonium bromide compounds form insoluble…

Polyvinyl Alcohol (PVA) is a synthetic, biocompatible and toxicologically safe polymer. Polyvinyl alcohol is used for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. It is useful as an anti-fading agent. It has various pharmaceuticle applications. In tablet…

Tryptic Soy Broth is a highly nutritive and very versatile medium recommended for general use in the laboratory. The medium promotes abundant growth of fastidious microorganisms without the necessity of adding serum and other materials. Tryptic Soy Broth is used for the cultivation of fastidious…

Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts.…

Agarose is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marinealgae. As a gelling agent, agarose is used: 1.) to separate nucleic acids electrophoretically because…

Sodium metasilicate nonahydrate is white powder, mainly used in detergent/washing industry, metal cleaner and ceramic industry. In detergent/washing industry, it can be used instead of Sodium triphosphate to raise cleaning efficiency and reduce enviroment pollution, provide excellent alkaline…

Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins, such as inclusion bodies, and be used for the recovery of periplasmic proteins. This can be used as the first…

MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable,…

Lecithin is a purified, wax-like phosphatidylcholine of soybean origin. In addition to being the major structural component of cellular membranes, phosphatidylcholine serves as a reservoir for several lipid messengers, it is the source of the bioactive lipids lysophosphatidylcholine, phosphatidic…

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The…

BRIJ 35 is a non-ionic detergent. BRIJ 35 is useful for the extraction of membrane proteins. For use in Stein-Moore Chromatography and all molecular biology applications. Typically used in the range of 0.1-1%.

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the…

Chloramine T Sodium Salt Trihydrate is a reagent for selective oxidation of methionine. It is used as an antibacterial agent; a topical antiseptic. Capable of oxidative cyclization to produce various heterocycles. Nitrene source for aziridinations and aminohydroxylations. Reactant for Preparation…

Benzalkonium Chloride is a quaternary ammonium salt. It is a cationic surface active agent and germicide. It is not effective against gram-negative bacteria or picornaviruses and it is not recommended for treatment of sanitizing critical or semicritical medical instruments. It has been reported…

Alginic acid sodium salt has been used in the flocculation of solids in water treatment; as sizing agent; thickener; emulsion stabilizer; and a suspending agent. It is used in the preparation of colloidal biodegradable structures such as gels, biofilms, beads, nanoparticles, and microcapsules…

Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. Allows recovery of active DNA after separation, with minimal inhibition of restriction and ligation enzymes Suitable for both analytical and preparative electrophoresis procedures Good…

Hexamethylenetetramine is a tetraamine compound with a structure analogous to adamantane. Hexamethylenetetramine is used for amino acid preparation. It is also used in synthesis as a formylation agent and is employed in preparing specialized materials. It is also used as a precipitating agent. …

Caffeine is a well-known drug commonly used as a mild stimulant, found in dietary sources such as coffee, tea, and cocoa. A central nervous system stimulant believed to act through adenosine receptors and monoamine neurotransmitters. It is an adenosine receptor antagonist and adenosine…

Agar is a polysaccharide complex extracted by bleaching and hot water treatment of agarocytes from the red alga Rhodophyceae, and usually consisting of the genera Gelidium, Acanthopeltis, Ceramium, Pterocladia and Gracilaria. The algae are typically found in the Pacific and Indian Oceans and in the…

Tricine, a zwitterionic buffer, was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. The name tricine comes from tris and glycine from which it was derived. Tricine can be used in…

Finely granulated, dry white powder prepared from agar. Agarose is the most popular medium for immunoelectrophoresis because of the large pore size for rapid diffusion and for low background staining by Coomassie Blue G stain. The low EEO and high gel strength is specifically selected and tested…

Polyvinyl Alcohol (PVA) is used as a tissue stabilizer or mounting media in histochemistry. PVA has been used as a preservative. PVA forms microgels in aqueous solution. Polyvinyl alcohol is used as a biological reagent to test the dispersion of zinc oxide nanoparticles. It is an effective protein…

Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand and a chelating agent. Ethylenediamine Tetraacetic Acid is a chelator of divalent cations. It also inhibits enzymes, such as metalloproteases, that require divalent cations for activity. Zinc-dependent…

Ferrous sulfate heptahydrate is a reagent used in the manufacturing of iron compounds, in electroplating baths, aluminium etching, process engraving and lithography, and in redox polymerisation. It is used as a reducing agent in chemical processes, leather dyes and writing inks. Also, it is a…

Cellulose is an organic compound, a polysaccharide consisting of a linear chain of several hundred to over ten thousand β(1→4) linked D-glucose units. Cellulose is used as bulking agent, opacifier, anti-caking agent, extrusion aid and stabilizer for foams and emulsions. High purity…

MOPS is used as a biological buffer. MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4. A buffer using MOPS sodium salt can be…

Thiamine (Vitamin B1) is one of the essential vitamins and is required for carbohydrate metabolism. Thiamine hydrochloride is used as a food-additive to add a brothy/meaty flavor to gravies or soups. It is also used as food supplement. Used in fluorometric determination of mercury.

Yeast extract powder is a vacuum dried autolyzed yeast extract produced from 100% Baker's yeast. It is a concentrated source of soluble protein components, carbohydrates, and B Complex vitamins natural to yeast. Yeast extract is used as a bacteriological media component for a variety of…

Polyvinyl alcohol (PVOH, PVA, or PVAl) is a water-soluble synthetic polymer. Polyvinyl alcohol is the raw material to make polymers. Polyvinyl alcohol is used as an emulsion polymerization aid, as protective colloid, to make polyvinyl acetate dispersions.

Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and…

MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. Concentrations higher than 20 mM should not be used with mammalian cell cultures.

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…