500 mL, bioWORLD
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Acrylamide and bis-acrylamide are major ingredients in polyacrylamide gels, a commonly used matrix for electrophoretic separation of nucleic acids and proteins. Fragments of DNA produced by various molecular biology techniques (eg. restriction endonuclease digestion, dideoxy sequencing, or the…
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Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME), containing four-fold concentrations of the amino acids and vitamins found in BME, as well as additional supplementary components. The original formulation of DMEM contained 1000mg/L of glucose, but…
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TBS + 1% Tween (10X)
bioWORLDBlot blocking buffer used to block non-specific binding site for antibodies on the membrane. 1% Tween-20 in 10X TBS
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IBS
bioWORLDIBS/Casein (Thomas Nos. C994C53 and C994C54) All blocks and diluents are supplied in a 10X concentration of physiological saline buffer. Sold as 2 x 100mL or 500mL. Technical Information: pH range: 7.0 (+/- 0.1) based upon an Imidazole physiologic buffer …
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All bioWORLD running buffers are prepared with high quality reagents.
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Magnesium Chloride, 1M, steril
bioWORLDAn excellent magnesium ion source for synthesis and biology research 1M Magnesium Chloride solution Sterile Common biological buffer Biotechnology Grade Nuclease-free Very hygroscopic Sterile, nuclease-free buffers and reagents have been prepared with the finest…
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Phosphate Buffered Saline
bioWORLDUltra Pure Grade pH at 25°CC: 7.4+/- 0.1 DNase Activity: None Sterility: Passes Storage: RT Hazard: Irritant
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MES Buffer
bioWORLDAlso known as [2-(N-Morpholino) ethanesulfonic Acid]. MES-Buffer has a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. A biological buffer mainly used in plant cell cultures. Used for culture media. Ultra Pure Grade …
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Tween 20
bioWORLDSynonyms: Polysorbate 20; Sorbitan Monolaurate Non-ionic nondenaturing detergent used in buffers to solubilize membrane proteins Commonly used in gels for PAGE isoelectric focusing Reagent Grade Heavy metals (such as Pb):
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DNA purification buffer using CTAB. Suitable for extraction of fungal DNA. Contains: 100mM Tric-Cl, pH 9.5 2% CTAB 1.4M NaCl 1% PEG 6000 or 8000 20mM EDTA RNAse and DNAse free Reference: Carlson JE, Tulsieram LK, Glaubitz JC, Luk VMK, Kauffeldt C, and Rutledge R (1991)…
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BBS/Casein
bioWORLDTechnical Information: 10X Concentrate has a working pH of 8.5 ( +/- 0.1) Formulated in a borate buffered physiologic saline buffer Sold as 2 x 100mL or 500mL.
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DMSO
bioWORLDACS Grade Freezing point: <18.30°C Residue after evaporation: <0.01% Water: <0.1% A275 (undiluted): <0.20 Titratable acid: <0.001meq/g Substances darkened by potassium hydroxide Caution: Readily absorbed through skin Appearance: Liquid …
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MOPS-EDTA 10X solution
bioWORLD10X solution of MOPS Buffer prepared with DEPC-treated double distilled H2O For RNA applications A buffer for RNA electrophoresis in denaturing (formaldehyde) agarose gels Contains: 0.4M MOPS, 0.1M Sodium Acetate, 0.01M EDTA Biotechnology Grade RNase activity:…
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PBS
bioWORLDPBS/Casein (Thomas Nos. C994C57 and C994C58 ) Supplied in a 10X PBS pH range 7.4 (+/- 0.1) Sold as 2 x 100mL or 500mL. PBS/Fish Gelatin (Thomas Nos. C994C59 and C994C60 ) Phosphate Buffered Saline/fish gelatin 0.05% blocks, diluents, and coating…
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HEPES Buffer
bioWORLDBiotechnology Grade DNase(P/F): None pH : 7.4 ±0.1 @ 25°C Protease(P/F): None RNase(P/F): None
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A.C.E. Acetate Buffer, 50 mM
bioWORLDThese convenient 50 mM premixed and ready-to-use A.C.E.™ Phosphate Buffers have been specially formulated for Capillary Electrophoresis. They are ideal for the separation of various biomolecules including proteins. Manufactured with the highest quality reagents in nucleasefree water, AMRESCO…
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Tween 80
bioWORLDA non-ionic nondenaturing detergent used in buffers to solubilize membrane proteins. Commonly used in gels for PAGE isoelectric focusing. Reagent Grade Heavy metals (such as Pb): Also known as Sorbitan Monooleate
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Calcium Chloride
bioWORLDIt has several common applications such as brine for refrigeration plants, ice and dust control on roads, and in concrete. The anhydrous salt is also widely used as a desiccant, where it will adsorb so much water that it will eventually dissolve in its own crystal lattice water. It can be…
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The bioWORLD bioPLUS™ pH calibration kit and pH standard buffer is suitable for laboratory and research use. Application: For Research & Laboratory Use Only
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Bradford Reagent, Ready to Use
bioWORLDBradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…
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IEF (Isoelectrofocusing) Buffers
bioWORLDThomas No. C995V08-V09 bioWORLD running buffers are prepared using high quality reagents so you can be asured of accurate test results Technical Information: Dilute with deionized water to make 1X 1X Concentration Contains: 7 mM Phosphoric Acid pH: 2.4 Thomas No. C995V10-V11…
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PBS with Calcium and Magnesium
bioWORLDWashing buffer for peroxidase conjugates in Western Blotting. 1X PBS with Ca++ and Mg++ pH 7.4 0.1 (25°C)
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bioPLUS™ Tris Buffer
bioWORLDThe bioWORLD bioPLUS™ tris buffer is suitable for use in biological and biochemical experiments. It is mainly used in gel electrophoresis. Application: For Research & Laboratory Use Only
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Acrylamide and bis-acrylamide are major ingredients in polyacrylamide gels, a commonly used matrix for electrophoretic separation of nucleic acids and proteins. Fragments of DNA produced by various molecular biology techniques (eg. restriction endonuclease digestion, dideoxy sequencing, or the…
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