500 mL, bioWORLD
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DMF (N',N-Dimethylformamide)
bioWORLDReagent grade A strongly polar organic solvent Refractive index: 1.423- 1.431 Hazardous Materials Description: N,N-Dimethylformamide Hazard Class or Division: 3 Identification Numbers: UN2265 Packaging Group: III *Applies additional hazardous materials handling…
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MES Buffer
bioWORLDAlso known as [2-(N-Morpholino) ethanesulfonic Acid]. MES-Buffer has a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. A biological buffer mainly used in plant cell cultures. Used for culture media. Ultra Pure Grade …
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Formaldehyde (4%)
bioWORLDCommon fixitive reagent for histology. Biotechnology Grade Contents: 4% formaldehyde in PBS
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PBS with Calcium and Magnesium
bioWORLDWashing buffer for peroxidase conjugates in Western Blotting. 1X PBS with Ca++ and Mg++ pH 7.4 0.1 (25°C)
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bioPLUS™ Water, DEPC-Treated
bioWORLDIt contains one oxygen and two hydrogen atoms connected by covalent bonds. It is commonly used in chemical reactions as a solvent or reactant and less commonly as a solute or catalyst. Application: For Research & Laboratory Use Only
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TAE Buffer, 50X Liquid Concentrate
bioWORLDTris-Acetate-EDTA (TAE) buffer, 50X A common buffer for electrophoresis A 50X solution of tris, acetate, and EDTA in distilled water
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DNA purification buffer using CTAB. Suitable for extraction of fungal DNA. Contains: 100mM Tric-Cl, pH 9.5 2% CTAB 1.4M NaCl 1% PEG 6000 or 8000 20mM EDTA RNAse and DNAse free Reference: Carlson JE, Tulsieram LK, Glaubitz JC, Luk VMK, Kauffeldt C, and Rutledge R (1991)…
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IEF (Isoelectrofocusing) Buffers
bioWORLDThomas No. C995V08-V09 bioWORLD running buffers are prepared using high quality reagents so you can be asured of accurate test results Technical Information: Dilute with deionized water to make 1X 1X Concentration Contains: 7 mM Phosphoric Acid pH: 2.4 Thomas No. C995V10-V11…
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PBS
bioWORLDPBS/Casein (Thomas Nos. C994C57 and C994C58 ) Supplied in a 10X PBS pH range 7.4 (+/- 0.1) Sold as 2 x 100mL or 500mL. PBS/Fish Gelatin (Thomas Nos. C994C59 and C994C60 ) Phosphate Buffered Saline/fish gelatin 0.05% blocks, diluents, and coating…
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Acrylamide and bis-acrylamide are major ingredients in polyacrylamide gels, a commonly used matrix for electrophoretic separation of nucleic acids and proteins. Fragments of DNA produced by various molecular biology techniques (eg. restriction endonuclease digestion, dideoxy sequencing, or the…
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Safranin-O-Counterstain
bioWORLDSafranin O is a biological stain commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. Safranin O has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure Safranin O is used to stain for chromosomes. In late Palaeozoic pteridosperm…
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HEPES Buffer
bioWORLDBiotechnology Grade DNase(P/F): None pH : 7.4 ±0.1 @ 25°C Protease(P/F): None RNase(P/F): None
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The bioWORLD bioPLUS™ pH calibration kit and pH standard buffer is suitable for laboratory and research use. Application: For Research & Laboratory Use Only
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EDTA, 0.5M sterile
bioWORLDA metal chelating agent commonly used in tissue culture media and biological buffers. Inactivator of nucleases. Prepared with DEPC for RNA applications. Biotechnology Grade C10H14N2O8Na2*2H2O CAS 60-00-4 MW 372.24 …
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LeiboVitz's L-15
bioWORLDLeibovits's L-15 Media was formulated to support the growth and proliferation of several cells lines in CO2 free systems without a sodium bicarbonate supplement. Whereas most cell culture media is buffered by sodium bicarbonate, L-15 is buffered by its salts, free base amino acids and…
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Phosphate Buffered Saline
bioWORLDUltra Pure Grade pH at 25°CC: 7.4+/- 0.1 DNase Activity: None Sterility: Passes Storage: RT Hazard: Irritant
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CDI-activated Separopore® Agarose6B
bioWORLDPre-activated medium for immobilization of ligands containing primary amines N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the…
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Medium 199 w/Earles Salt w/L-Glut
bioWORLDIn an effort to produce a totally well-defined nutritional source for cells, Morgan, Morton and Parker developed what has become known as Medium 199. It was found the growth of explanted tissue could be measured in Medium 199. However, long-term cultivation of cells required a serum supplement.…
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Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…
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All bioWORLD running buffers are prepared with high quality reagents.
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Calcium Chloride
bioWORLDIt has several common applications such as brine for refrigeration plants, ice and dust control on roads, and in concrete. The anhydrous salt is also widely used as a desiccant, where it will adsorb so much water that it will eventually dissolve in its own crystal lattice water. It can be…
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Gelatine-Blocking Buffer
bioWORLDBlot blocking buffer used to block non-specific binding site for antibodies on the membrane. 1% Gelatin in PBS
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Tween 20
bioWORLDSynonyms: Polysorbate 20; Sorbitan Monolaurate Non-ionic nondenaturing detergent used in buffers to solubilize membrane proteins Commonly used in gels for PAGE isoelectric focusing Reagent Grade Heavy metals (such as Pb):
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Glycine buffer 0.2M, (pH 3.0)
bioWORLDCommon buffer for biological sciences. A compound used for polyacrylamide gel electrophoresis and Western blots Biotechnology Grade A280 (1.0M, water) <0.1 Chromatography: Single spot Heavy metals (as Pb) <0.002% Appearance: Liquid Boiling Point:…
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Acetate Buffer, 1M
bioWORLDCommon buffer for biological sciences. Ultra Pure Grade 1M Concentration Appearance: Liquid Melting Point: 323-329 °C PH Value: 4.0 RTECS Number: AJ4375000
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Formamide, deionized
bioWORLDIt is a clear liquid amide derived from formic acid and can be used as cryoprotectant and gel-stabilizer. It is known to destabilize nucleic acid duplexes, making it useful for preparing hybridization buffers for RNA and DNA applications. Conductivity (µmhos): <100 Abs. @ 280nm:…