Used in coupling of hydroxy, amino or thiol groups to Separopore® 6B via a 12-carbon hydrophilic spacer arm. Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group. Ideal for immobilization of alkali-resistant small ligands such…

1 grams make about 10ml gel Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag 1ml drained gel binds 5-10mg of recombinant GS-protein

The method involves alkylation of the parent nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N 6 - carboxymethyl derivative with ubsequent condensation using 1,6 diaminohexane to give N 6 -[(6-aminohexyl)carbamoylmethyl)]. Specifications: …

Applications: Cyanogen bromide-activated Separopore® 6B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore®…