Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

a-Lactose-Separopore® 4B-CL is used in protein chromatography, affinity chromatography and carbohydrate matrices. a-Lactose-Separopore® 4B-CL has been used to study microneme proteins and host-parasite cell surface receptors. Lactose-Separopore® 4B-CL may be used to isolate lactose binding…

Silica-coated uniform superparamagnetic beads, MagneZoom, with an aldehyde surface group. It is widely used to covalently couple ligands containing primary and secondary amino groups. This coupling method will provide an extremely stable linkage between the ligand and the matrix. Size: 2-5µm …

bio-World's Carboxy Coupling Kit consists of amino-terminated Separopore® 4B-CL (4% crosslinked agarose beads). This kit is suitable for covalent immobilization of peptides or other carboxyl-containing (–COOH) molecules for use in affinity purification procedures. It includes carboxyl Coupling…

Fucose-Separopore® 4B-CL used as affinity matrix for the purification of fucose-binding glycoproteins. Fucose is linked to Separopore® 4B-CL via a spacer arm to ensure efficient binding of this ligand to the protein.

Heparin Separopore® 4B is an affinity matrix widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid receptors. It can also be used as a high…

An affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP).  Amylose is linked to Separopore® (Agarose) 4B-CL. The resin may be generated up to five times.  Store used resin in column buffer containing 0.02% sodium azide. Quantitative Analysis: Protein…

Galactose coupled Separopore® is a high strength bioagarose matrix. Galactose-Separopore® 4B-CL is used in the purification of agglutinins, toxins, lectins, galactosidases and other galactose-binding molecules, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins. This…

Silica-coated uniform super paramagnetic beads with surface carboxyl group. Proteins and nucleic acids can be covalently bound to these beads with the retention of biological activity. Size: 5-10µm. COOH terminated Binding: up to 20mg/ml. pH stability: 4-9 Supplied as 25mg/ml…

N-Acetyl-glucosamine-Separopore® 4B-CL is used to purify wheat germ (Triticum vulgaris) lectin, other lectins, and certain other carbohydrate-binding proteins. The purification results in specificity and prolonged biological activity of the protein depending on the applied conditions.…

N-Acetyl-D-galactosamine-Separopore® 4B-CL is an agarose affinity chromatography matrix used to purify carbohydrate-binding proteins such as membrane receptors, and also lectins with affinity to N-Acetyl-galactosamine. N-Acetyl-D-galactosamine-Separopore® 4B-CL has been used to study periodontal…

Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides…

Rapid immobilization of antibodies and other proteins to the matrix Immobilization of ligands through primary amines to purify recombinant proteins Immobilization of Protein A or Protein G for monoclonal antibody purification Pre-activated medium for immobilization of ligands containing a…

bio-World provides immobilized E. coli proteins on a solid bio-affinity support for the removal of antibodies to E. coliproteins from antisera prepared against recombinant proteins expressed in E. coli. Our immobilized E. coli lysate consists of partially purified proteins from E. coli strains…

Gelatin Separopore® 4B has been designed for the purification or removal of fibronectin. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma.

Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore® 4B). This matrix is coupled through a stable, leak resistant linkage over a wide pH range. May also be used to immunoprecipitate…

Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Co metal…

Protein G binds specifically to Fc regions of many mammalian immunoglobulins Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…

Metal ion-chelating Separopore® 4B-CL is designed for the affinity purification of 6X Histidine-tagged proteins. Nickel, Cobalt, Copper and Zinc are used as cations for this type of purification because they provide effective binding and selectivity for a wide range of proteins. Affinity matrix for…

bioSilica Beads Non-porous silica beads are useful in molecular biology and immunoassays. Diameters are 0.3-5.0 µm, a density of 1.96g/cm 3 , a refractive index (589nm) of about 1.37. Silica beads have high temperature stability. Also, because hydrophilic silica does not absorb proteins,…

Affinity matrix with immobilized KLH used for the purification of antibodies generated against peptide-KLH conjugated antigens byremoving antibodies against the KLH portion of the antigen. Anti-KLH antibodies can be removed by solid phase immuno-affinity column chromatography over the KLH-…

Papain-Separopore® 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce Fab fragment of IgG antibodies. It is also…

Erythrina cristagalli (coral tree) lectin is a 54,000 dalton glycoprotein consisting of two different subunits of approximately 28,000 and 26,000 daltons. It has a specificity toward galactose residues and appears to have the highest binding activity toward galactosyl ( b -1,4)…

Applications: Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with…

Applications: Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to this immobilized ligand. The term "Thiophilic" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic adsorption…

Standard used for determining protein concentration by CB protein assay. This coomassie dye-based protein assay provides simple, rapid estimation of protein concentration and can be performed using as little as 1mg protein per sample. CB-Protein Assay is supplied ready to use with an easy…

Applications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from…

Applications: Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in…

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Ni-Chelated Separopore® 4B is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Imminodiacetate immobilized matrix charged with Ni metal ions. IIdeal for…

Applications: Reduced glutathione is covalently linked Separopore® 4B Microbeads for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…