Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first…

* Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins * Used in the isolation of RNA * Solubilizes insoluble or denatured proteins such as inclusion bodies * Can be used for the recovery of periplasmic proteins

Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds, it is a strong chaotropic agent. Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a free flowing powder…