MilliporeSigma, UN 3245
-
When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
-
The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types…
-
MISSION(R) TRC3 ORF BFP Lentivirus High Titer Control
MilliporeSigmaThe MISSION TRC3 ORF BFP Lentivirus High Titer Control Transduction Particles contain an ORF insert expressing BFP, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome…
-
When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
-
HepaRG(TM) Cells MDR3 (-/-) one vial
MilliporeSigmaHepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MDR3 knockout cells…
-
When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
-
The transcriptional activator VP64 fused to nuclease-dead SpCas9 (dCas9), forms a ribonucleoprotein complex (RNP) with the CRISPR guide RNA (gRNA). The stem- and tetra-loop sequences in the gRNA scaffold have been modified into minimal hairpin RNA aptamers, which selectively bind dimerized MS2…
-
Sigma(R) is proud to offer IPTG-inducible vectors as the latest development from our continued partnership in TRC.The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG…
-
This product is a kit containing two sets of ready to use lentiviral particles enabling immediate transduction for strong expression in a wide range of both dividing and non-dividing cell lines. MS2-P65-HSF1 lentiviral particles efficiently and stably integrate MS2-P65-HSF1 and hygromycin…
-
When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
-
Developed by Feng Zhang's lab at the Broad Institute, the mouse GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the mouse genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2…
-
The Sigma CRISPR-LENTI HUMAN HPRT1 POSITIVE CONTROL TRANSDUCTION PARTICLES are a critical positive control to monitor transduction efficiency for lentiCRISPRs. This control is produced from the sequence-verified CRISPR lentiviral plasmid targeting human HPRT1. The protein encoded by this gene is a…
-
MISSION(R) Lenti microRNA, Human hsa-let-7i-5p
MilliporeSigmaSigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the…
-
-
-
When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
-
The SIG10 5a Chemically Competent Cells share the most useful genetic elements of standard cloning strains like DH5alpha(TM) and directly replace them in cloning protocols. They provide the performance researchers need with ease of use as only common laboratory equipment is required and provide…
-
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG AHR knockout cells express…
-
OverExpress(TM) Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been…
-
MISSION(R) Lenti microRNA, Human hsa-miR-15a-5p
MilliporeSigmaSigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the…
-
The Lenti CRISPR HPRT1 gRNA Control Transduction Particles (LV02 vector) product is a critical positive control used to monitor transduction efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with…
-
MISSION(R) Lenti microRNA, Human hsa-miR-183-5p
MilliporeSigmaSigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the…
-
MISSION(R) Lenti microRNA Inhibitor, Mouse mmu-miR-375-3p
MilliporeSigmaIndividual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a…
-
MDR1/MRP2 Double Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and MRP2 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional double knockout of the ABCB1 and ABCC2 (MDR1/MRP2) efflux transporters. Synonyms: …
-
Cas9 Neomycin Lenti Particles
MilliporeSigmaReady-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Neomycin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Neo using the…
-
Cas9 GFP Lentiviral Particles
MilliporeSigmaReady-to-use Cas9-GFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-GFP lentiviral particles efficiently and stably integrate Cas9 and GFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing…
-
XLDNA SIG10 Electrocompetent cells for BAC & cosmid cloning
MilliporeSigmaThe XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields. These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct…
-
BCRP Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCG2 (BCRP) efflux transporter. Synonyms: C2BBe1 Cells BCRP (-/-/-/-) …
-
OverExpress(TM) Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been…
-
MISSION(R) TRC3 ORF GFP Lentivirus High Titer Control
MilliporeSigmaThe MISSION TRC3 ORF GFP Lentivirus High Titer Control Transduction Particles contain an ORF insert expressing TurboGFP(TM), and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole…
-
MISSION(R) Human Kinase Lentiviral shRNA Pool
MilliporeSigmaSigma shRNA Human Kinase Pooled Lentiviral LibrarySigma-Aldrich has a long-standing expertise in pooled lentiviral screening spanning whole genome and custom pool offerings. Due to the popular demand of the kinase gene panel, we are proud to offer an off-the-shelf, ready-to-use shRNA kinase pool…
-
Developed at the Broad Institute by Feng Zhang's lab, the human GeCKO v2 (two vector system) libraries consist of over 100,000 unique gRNAs for gene knock-out in the human genome. Using a dual lenti CRISPR vector wherein the pooled libraries will only express the gRNA (with the lentiGuide-Puro…