This product is a kit containing two sets of ready to use lentiviral particles enabling immediate transduction for strong expression in a wide range of both dividing and non-dividing cell lines. MS2-P65-HSF1 lentiviral particles efficiently and stably integrate MS2-P65-HSF1 and hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin). dCas9-VP64 lentiviral particles efficiently and stably integrate dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin). They are ideal for avoiding the tedious lentivirus production process and are part of a three part CRISPR system with individual dCas9-VP64. MS2-p65-HSF1 and gRNA expression vectors.To order gRNA in any format click here
Storage: -70C
Application: Functional Genomics/Target Validation
•Unbiased forward genetic screening
•Strong transcriptional activation in multiple cell lines
•Creation of cell lines stably expressing dCas9-VP64 and MS2-p65-HSF1.
Legal Information: CRISPR Use License AgreementLentiviral, WPRE and Evrogen Fluorophore Licenses