emp Biotech
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CentriPure Gel Filtration Columns
emp BiotechCentriPure Gel Filtration Columns are designed for rapid desalting and efficient removal of small molecules (dyes, salts, buffers, biotin, haptens, etc.) from proteins and nucleic acids.
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For each synthesis cycle, up to 1 to 2 % of free 5’-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent „Capping“ step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain…
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CentriPure Gel Filtration Columns
emp BiotechCentriPure Gel Filtration Columns are designed for rapid and efficient removal of small molecules such as dyes, salts, biotin, ammonia, haptens, etc. from larger nucleic acids, proteins or nanoparticles, which are simultaneously purified and desalted in a single step.
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Cleavage of the oligonucleotide from its solid support and subsequent removal of all protecting groups from the nucleobases and phosphates close the cycle of automated oligonucleotide synthesis and bring it to completion. For this purpose, three different Cleavage solutions from emp BIOTECH are…
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Hyacinth BMT activator (also known as 5-Benzylmercapto-1H-tetrazole or BTT) demonstrates important advantages in the syntheses of oligonucleotides: Coupling efficiencies of 99% with impeccable quality Lower percentage of n - 1 sequences Dramatic reduction of coupling times to under 3…
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4,5-Dicyanoimidazole (DCI) is an efficient activator for use in chemical synthesis of DNA. 2.5L, GL45 thread
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