Cayman Chemical, 96 Well
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Glutathione Assay Kit
Cayman ChemicalCayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative…
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Alanine Transaminase Colorimetric Activity Assay Kit
Cayman ChemicalALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and…
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Glycogen Assay Kit
Cayman ChemicalGlycogen is the principal storage form of glucose in animal and human cells. It is made primarily by the liver and muscles, but it can also be made by glycogenesis within the brain and stomach. Cayman’s Glycogen Assay provides a simple, reproducible, and sensitive tool for assaying glycogen…
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Leukotriene E4 ELISA Kit
Cayman ChemicalLeukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously…
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Triglyceride Colorimetric Assay Kit
Cayman ChemicalThe measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell…
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Anti-Ovalbumin IgE (mouse) ELISA Kit
Cayman ChemicalImmunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that…
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11-keto Testosterone ELISA Kit
Cayman Chemical11-KT is a second key androgenic steroid found in fish. It occurs in males together with testosterone in amounts which vary from less than 1 ng/ml to as much as 50-100 ng/ml, depending on the species and the stage of the reproductive cycle.
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RARß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, beta (NR1B2), a ligand-dependent transcription factor commonly referred to as RARB or RARβ. INDIGO’s reporter cells…
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COX (ovine/human) Inhibitor Screening Assay Kit
Cayman ChemicalThe COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major PG compounds. Thus, the Cayman COX Assay is more accurate and…
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Aconitase Assay Kit
Cayman ChemicalAconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated…
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11ß-Prostaglandin F2a ELISA Kit
Cayman Chemical11β-PGF2α is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis. The normal human urinary excretion of 11β-PGF2α is about 11 ng/mmol creatinine (~400…
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MitoCheck® Complex II Activity Assay Kit
Cayman ChemicalComplex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to fumarate, and in the process reduces ubiquinone (Q) to ubiquinol (QH2). Ultimately,…
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MitoCheck® Complex II/III Activity Assay Kit
Cayman ChemicalComplex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration, and as a major source of reactive oxygen species III. It accepts electrons from complexes I and II in the form…
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Phosphatidylcholine Colorimetric Assay Kit
Cayman ChemicalCayman’s PC Assay provides a specific, sensitive, and convenient method for quantifying PC in plasma or serum. In this assay, PC-Specific PLD hydrolyzes PC to choline and phosphatidic acid. The newly formed choline is then used to generate hydrogen peroxide in a reaction catalyzed by choline…
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VDR (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human vitamin D receptor (NR1I1), a ligand-dependent transcription factor commonly referred to as VDR. INDIGO’s reporter cells include the luciferase reporter…
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Nitrate/Nitrite Colorimetric Assay Kit (LDH method)
Cayman ChemicalNADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH used in a…
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Creatinine (urinary) Colorimetric Assay Kit
Cayman ChemicalUrinary creatinine levels are commonly used as an index of standardization for a variety of other tests. Measurement of creatinine clearance is also useful in detecting renal disease and estimating the extent of impairment of renal function. The Cayman Chemical Creatinine (urinary) Colorimetric…
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Pyruvate Assay Kit
Cayman ChemicalPyruvate (pyruvic acid) is a key intermediate in cellular metabolic pathways and is derived primarily from glucose via glycolysis. Abnormal blood pyruvate levels are reported in a number of disorders including shock, liver disease, congestive heart failure, diabetes mellitus, thiamine deficiency,…
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Interleukin-6 (mouse) ELISA Kit
Cayman ChemicalInterleukin-6 (IL-6) is produced by monocytes, macrophages, and T cells and is invovled in many defense mechanisms including the immune response, hematopoiesis, bone metabolism, and acute phase reactions. Cayman’s IL-6 (mouse) ELISA Kit is an immunometric (i.e., sandwich) ELISA that permits…
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Oxygen Consumption Rate Assay Kit (MitoXpress® Xtra HS Method)
Cayman ChemicalThe oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from healthy oxidative phosphorylation to aerobic glycolysis in cancer cells. Oxygen…
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Acylated Ghrelin (mouse, rat) Easy Sampling EIA Kit
Cayman ChemicalFor the measurement of acylated ghrelin.
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Anti-Citrullinated Human Fibrinogen Assay Kit (mouse)
Cayman ChemicalImmunization of mice with citrullinated human fibrinogen, especially mice expressing the human HLA-DR4 transgene, induces an arthritic response driven by the production of antibodies that recognize citrullinated epitopes. The polyclonal antibody response produces antibodies reactive with both…
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LXRß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human liver X receptor beta (NR1H2), a ligand-dependent transcription factor commonly referred to as LXRβ. INDIGO’s reporter cells include the…
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TR (human) Reporter Assays Panel
Cayman ChemicalINDIGO’s panel of TR reporter assays utilizes non-human mammalian cells engineered to express human thyroid hormone receptors alpha (NR1A1) and beta (NR1A2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. INDIGO’s TR reporter cells include the…
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Substance P ELISA Kit
Cayman ChemicalSubstance P is a bioactive 11-amino acid peptide first isolated in 1931 from brain and intestine. The peptide is involved in many physiological processes including pain modulation, smooth muscle contraction, blood pressure control, kidney function, and water homeostasis. Substance P is widely…
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SIRT3 Direct Fluorescent Screening Assay Kit
Cayman ChemicalThe sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman’s Direct Fluorescent Screening Assay Kits provide a convenient…
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Cayman Practice ELISA Kit
Cayman ChemicalThis assay has been developed for researchers that do not have experience performing ELISAs. It can be used as a practice tool allowing you to become comfortable with running Cayman ELISAs. This kit contains enough reagents to run at least four complete standard curves.
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TBARS (TCA Method) Assay Kit
Cayman ChemicalLipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds, which…
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8-Isoprostane ELISA Kit
Cayman ChemicalThe isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also…
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ß-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit
Cayman Chemicalβ-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is produced in the liver, mainly from the oxidation of fatty acids, and exported to peripheral tissues for use as an energy source. Measurement of β-HB can provide a reliable index of the level of normal ketosis as well as detect…
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Progesterone ELISA Kit
Cayman ChemicalProgesterone, along with pregnenolone, is the biosynthetic precursor of all other steroid hormones. The main function of progesterone is to prepare the uterine lining for implantation of a fertilized ovum and to maintain pregnancy. Measurement of serum or plasma progesterone levels are used as an…
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Aldehyde Dehydrogenase Activity Assay Kit
Cayman ChemicalAldehyde dehydrogenases (ALDHs) represent a group of enzymes that oxidize a wide range of endogenous and exogenous aldehydes to their corresponding carboxylic acids, which affect both developmental and toxicological functions. Pharmacological inhibitors or activators of ALDH activity have been…