Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides…

Applications: Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in…

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Applications: Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good…

Red-colored agarose matrix contains immobilized Streptavidin for convenient affinity capture (pull-down) of biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands. viewIP TM Red Affinity Gels greatly reduce the risk of pellet loss.…

Separopore® 4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 4B is a proven base matrix for coupling affinity ligands. Separopore® 4B provides good…

Separopore® 6B (macrobeads) is a larger sized agarose macrobead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

A complete kit to bind Protein A fusion proteins expressed in E. coli or yeast. Kit consists of affinity chromatography gel matrix, IgG-Separopore® 4B, including all the components/buffers that allow for quick, convenient purification of anti-IgG antibodies. This kit may also be used to…

Used for protein purification by batch/column and scale-up procedures. Glutathione Separopore ®  Super Flow (S F) has an exellent protein binding capacity. The good flow properties of the 92 μm (average size) highly cross-linked Separopore ® matrix makes an…

Application: Anti-BSA-Separopore® 4B is used for immunoprecipitation and immunoaffinity purification of bovine serum albumin (BSA) from solutions (and co-immunoprecipitation of biomolecules that bind BSA). It is also used to remove BSA from solutions.

Ni-Chelated Separopore® 4B is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Imminodiacetate immobilized matrix charged with Ni metal ions. IIdeal for…

Application: For the purification of avidin and streptavidin containing proteins.

1 grams make about 10ml gel Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag 1ml drained gel binds 5-10mg of recombinant GS-protein

Application: Poly-L-Lysine-Separopore® 4B-CL is used for the purification of negatively charged proteins and biomolecules.

Applications : Used for the purification of liposomal proteins and glycopeptide antibiotics such as teicoplanin and vancomycin group.

Applications: Phenyl-Separopore® 4B-CL is used for hydrophobic interaction chromatography to purify membrane proteins and other proteins with hydrophobic regions. This matrix separates proteins based on the basis of the different hydrophobicities of the proteins.

Applications: Reduced glutathione is covalently linked Separopore® 4B Microbeads for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

Applications: The red-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows…

Glutathione Separopore® HR is a high resolution matrix highly cross-linked 38 µm agarose bead (6%) matrix.Glutathione Separopore® HR ensures the lowest sample dilution and best high-resolution separation.

The method involves alkylation of the parent nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N 6 - carboxymethyl derivative with ubsequent condensation using 1,6 diaminohexane to give N 6 -[(6-aminohexyl)carbamoylmethyl)]. Specifications: …

Applications: Aldehyde Separopore® 4B has a robust reductive amino alkylation coupling chemistry to immobilize proteins and other ligands through primary amines (–NH2) to the resin. It can be used for a variety of affinity purification methods involving batch or column chromatography.…

Applications: Reactive Blue 4-Separopore is a dye affinity matrix used to purify proteins based on selective binding interactions between certain protein sites and the dye molecule. Differerent immobilized dyes bind from 5 to 60% of the proteins in various crude cell extracts. The affinity for…

Application: For use in immunoprecipitation and Co-immunoprecipitation studies. Affinity isolated antibody goat anti-rabbit IgG coupled to cyanogen bromide-activated Red Separopore ® offers the advantage of increased sensitiveity for rabbit IgG without cross-reactivity to other…

Cu-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Cu metal ions.v…

A dye-ligand affinity matrix consists of Reactive Green-19 bound to cross-linked 4% agarose beads (Separopore® 4B-CL).

Applications: Tosyl-activated Separopore® 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated Separopore® 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through…

Separopore® 4B (macrobeads) is a larger sized agarose bead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

Applications: Protein A Blue-Separopore® 4B-MB functions same as conventional Protein A affinity beads, but offers enhanced visibility due to blue colored Separopore® macrobeads. The blue colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in…

Zn-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Zn metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Zu metal…

Application: For the purification of membrane dicarboxylate binding proteins and other proteins with affinity to glutamate.

Applications: Cyanogen bromide-activated Separopore® 6B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore®…

Applications: Histidine-Separopore® 4B-CL can be used in the purification of supercoiled (sc) plasmid DNA (pDNA) from Escherichia coli lysates. This can also be used for the separation of IgG from human plasma and selective purification of bioactive oligouronides such as α-(1,…