bioWORLD, Affinity Chromatography
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bioWold's Streptalon (Streptavidin magnetic beads) Kit is designed for convenient and quick purification of biotinylated proteins and nucleic acids in immunoprecipitation, protein interaction studies, immunoassays and cell isolation, etc. Streptalon (Streptavidin Magnetic Beads) Kit…
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Biosilica™ beads
bioWORLDbioSilica Beads Non-porous silica beads are useful in molecular biology and immunoassays. Diameters are 0.3-5.0 µm, a density of 1.96g/cm 3 , a refractive index (589nm) of about 1.37. Silica beads have high temperature stability. Also, because hydrophilic silica does not absorb proteins,…
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Goat anti-Human IgG Nano-activeGold™
bioWORLDNano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™ reagents consist of affinity-purified IgG antibodies, goat anti-human IgG covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron…
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Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides…
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Carboxyl Coupling Kit, 10 mL
bioWORLDbio-World's Carboxy Coupling Kit consists of amino-terminated Separopore® 4B-CL (4% crosslinked agarose beads). This kit is suitable for covalent immobilization of peptides or other carboxyl-containing (–COOH) molecules for use in affinity purification procedures. It includes carboxyl Coupling…
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Protein-A Separopore® 4B
bioWORLDAffinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as pre-packed,…
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Erythrina cristagalli (coral tree) lectin is a 54,000 dalton glycoprotein consisting of two different subunits of approximately 28,000 and 26,000 daltons. It has a specificity toward galactose residues and appears to have the highest binding activity toward galactosyl ( b -1,4)…
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High quality microplates coated with a high binding heparin solution. Heparin, Sodium salt is a low molecular weight heparin (LMWH) polymer that produces its major anticoagulant effect by activating antithrombin. This activation causes a conformational change in ATIII and allows for increased…
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Ni-IMAC 1mL Columns
bioWORLDNi-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant…
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bioWorld's Carboxylated 96-well Microplates contains a COOH-coated surface to which the amino group present in any molecules, such as peptides or proteins, binds to through formation of amide bonds by the action of carbodiimide. Applications: Coupling molecules, having an amino group, to…
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MagneZoom™ CAM (Calmodulin)
bioWORLDCalmodulin covalently bound to MagneZoom magnetic beads. For purification of calmodulin-binding proteins and calmodulin-regulated proteins. Proteins containing a calmodulin binding peptide fusion tag can also be purified using MagneZoom - CAM. Particle Size: 2-5µm Ligand: Bovine Calmodulin …
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Jacalin, belonging to the lectins family, is a Hemagglutinin from the seeds of the jack fruit Artrocarpus integrifolia . It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with specific carbohydrate structures. Jacalin shows specific…
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Amylose Separopore® (Agarose)
bioWORLDAn affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). Amylose is linked to Separopore® (Agarose) 4B-CL. The resin may be generated up to five times. Store used resin in column buffer containing 0.02% sodium azide. Quantitative Analysis: Protein…
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Carboxy-terminated Magnetic Beads
bioWORLDSilica-coated uniform super paramagnetic beads with surface carboxyl group. Proteins and nucleic acids can be covalently bound to these beads with the retention of biological activity. Size: 5-10µm. COOH terminated Binding: up to 20mg/ml. pH stability: 4-9 Supplied as 25mg/ml…
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ConA-MagneZoom Kit is designed for quick and efficient small scale purification by magnetic separation of macromolecules containing a-D-mannosyl or a-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides. The kit includes paramagnetic beads of average 5 µm in diameter covalently…
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Zerobind™ Affinity Columns
bioWORLDZerobind™ affinity columns are designed to remove non-specific backgrounds from various polyclonal antibodies generally used for western blotting. Passing the diluted Antibody solution through Zerobind™ column can reduce non-specific binding while retaining activity against your…
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MagneZoom™A Protein-A Magnetic Beads
bioWORLDRecombinant protein-A covalently linked to MagneZoom (magnetic beads). For magnetic separation and isolation of IgG subclasses from ascites, serum, or cell culture supernatants. Particle size: 2-5µm Ligand: rProtein A Binding Capacity: 0.20mg rabbit IgG/ml beads pH stability: 4-9 …
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Ni-alginate macro and micro beads
bioWORLDBioalginate beads Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent…
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Streptavidin Coated Plates are available in 96-well plate format, and pre-blocked with BSA Blocking Buffer. bio-world Biotin-Binding Plates are available in clear to be used with colorimetricdetection methods. Easy and gentle immobilization of biotin-containing conjugates…
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Biotin Coated Plates can be used as the basis for a variety of biomolecular assay techniques requiring the immobilization of probes that are conjugated with NeutrAvidin Protein, streptavidin, avidin or other biotin-binding proteins. Specifications: Biotin group accessible for binding…
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Cu-alginate macro and micro beads
bioWORLDBioalginate beads Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent…
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Nickel-Coated Microplates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing…
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MagneZoom™-PNA (Peanut Agglutini)
bioWORLDMagneZoom-PNA are silica-coated super paramagnetic beads covalently linked to Peanut Agglutinin (PNA). Peanut agglutinin is a 110 kDa homotetrameric, non-glycosylated lectin with a high binding affinity for the tumor associated Thomsen-Friedenreich antigen (“T’-antigen) and binds preferentially to…
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Streptavidin Separopore® (Agarose)
bioWORLDApplications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…
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Protein-A Gold™
bioWORLDProtein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus . It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules,…
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Co-Chelated Separopore®
bioWORLDCo-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Co metal…
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Anti-biotin Goat IgG Nano-activeGold
bioWORLDNano-activeGold™ can be used in immunoblotting, light microscopy, and electron microscopy to provide clear visibility. Smallest commercially available gold immunoprobes. Penetrates and reaches antigens inaccessible to other probes. Extremely uniform 1.5 nm diameter gold label. …
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Cu-IMAC Columns
bioWORLDCu-immobilized metal affinity chromatography (IMAC) columns consist of Cu-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Cu-IMAC Columns separate proteins via their differences in affinity to Cu2+ metal ions. Ideal for purification of recombinant…
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Bioalginate beads
bioWORLDBioalginate Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent cations,…
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Epoxy Nanospheres MagneZoom™, 5 mL
bioWORLDSupplied as a 2.5% suspension MagneZoom™-Epoxy are silica-coated super paramagnetic beads with an Epoxy surface group. bioWORLD’s MagneZooms are suitable for high throughput screening. Matrix: Silica coated magnetic beads. Magnetic bead ligand: Epoxy. Particle size:…
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Protein A coated microplates provide alternative methods for immobilizing antibodies for ELISA and other plate-based assay techniques. These plates are uniformly and stably coated with Protein A. They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of…
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Wheat germ -Triticum vulgaris- agglutinin (WGA) linked to MagneZoom (magnetic beads). For magnetic separation by binding specifically to cell particles, glycoconjugates and polysachharides containing N-acetylglucosamine residues. Size: 2-5µm. Ligand: Wheat germ agglutinin Ligand…