i7™ Hot Start High-Fidelity DNA Polymerase has the high-fidelity, sensitivity and processivity with an error rate ~2.8×10^2 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes. Applications: Long and difficult template DNA…

Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity.…

i7™ High-Fidelity DNA Polymerase has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes. Applications: Long and difficult template DNA…

IgScript™ probe-based RT-qPCR kit combines two powerful mixtures: i). IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures…

i7™ High-Fidelity DNA Polymerase 2x master mix is a ready to use premix which contains high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer.  i7™ High-Fidelity DNA Polymerase 2x master mix has the high-fidelity,…

IgScript™ probe-based RT-qPCR kit combines two powerful mixtures: i). IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures…

Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps.  The advanced formulation of this kit also allows fast PCR cycling…

Intact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase…

Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps…

Intact Genomics igMax™ DH10B derivative ElectroCompetent cells are suitable for demanding cloning situations, such as synthetic bio-applications, BAC cloning, assembling large and multi-DNA fragments, and large construct transformation or cloning difficult targets, requiring the greatest…

Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration…

T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA (1). The mode of action of T5 Exonuclease in vivo may be…

UvsY is the phage T4 recombination mediator protein, and structural and biophysical studies provide insights into its role in T4 homologous recombination. During T4 homologous recombination, the UvsX recombinase must compete with the prebound gp32 single-stranded binding protein for DNA-binding…

Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat…

Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. T4 UvsX DNA…

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. Intact Genomics Hot Start Taq 2x Master Mix has been optimized for routine PCR applications. Hot start Taq is a thermostable…

Intact Genomics ElectroCompetent Agrobacterium Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research.  The ElectroCompetent Agrobacterium Combo Pack contains 3 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia…

Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease…

T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli . Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form…

Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression. Product Includes: BL21 (DE3) competent cells pUC19 control DNA Recovery medium

Intact Genomics Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the…

Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ TG1 Phage Display chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by…

igScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction…

Intact Genomics chemically competent DL39 (DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues.  DL39 (DE3) can also be used to reduce NMR cross-labeling via transaminase activity for…