Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand. Ethylenediamine tetraacetic acid (EDTA) is a calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals. For use as an anticoagulant, disodium or tripotassium…

Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested. …