Chemicals, UN 3245
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MISSION(R) Lenti microRNA, Human hsa-miR-146a-5p
MilliporeSigmaSigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the…
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HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG AHR knockout cells express…
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HepaRG(TM) 5F Control Cells 1 vial
MilliporeSigmaThis product consists of ZFN engineered HepaRG 5F clone control cells. They are intended for use as both control cells for HepaRG(TM) knockout cells as well as for a wide variety of liver cell based assays. Hepa RG cells display hepatocyte-like functions and can be a replacement to primary human…
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OverExpress(TM) Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been…
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The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types…
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Cas9 GFP Lentiviral Particles
MilliporeSigmaReady-to-use Cas9-GFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-GFP lentiviral particles efficiently and stably integrate Cas9 and GFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing…
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Developed by Feng Zhang's lab at the Broad Institute, the human GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the human genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2…
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The Lenti CRISPR Non-Target Control#3 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#3 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types…
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Developed by Feng Zhang's lab at the Broad Institute, the mouse GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the mouse genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2…
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Kidney PTEC Control Cell (SA7K Clone)
MilliporeSigmaSigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma's RPTEC's exhibit normal epithelial…
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Cas9 RFP Lentiviral Particles
MilliporeSigmaReady-to-use Cas9-RFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-RFP lentiviral particles efficiently and stably integrate Cas9 and RFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing…
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MISSION(R) TRC3 ORF BFP Lentivirus Control
MilliporeSigmaThe MISSION TRC3 ORF BFP Lentivirus Control Transduction Particles contain an ORF insert expressing BFP, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome LentiORF…
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OverExpress(TM) Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been…
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MRP2 Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP2 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC2 (MRP2) efflux transporter. Storage: -196C Application: The…
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MISSION(R) Human Kinase Lentiviral shRNA Pool
MilliporeSigmaSigma shRNA Human Kinase Pooled Lentiviral LibrarySigma-Aldrich has a long-standing expertise in pooled lentiviral screening spanning whole genome and custom pool offerings. Due to the popular demand of the kinase gene panel, we are proud to offer an off-the-shelf, ready-to-use shRNA kinase pool…
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The SIG10 5a Chemically Competent Cells share the most useful genetic elements of standard cloning strains like DH5alpha(TM) and directly replace them in cloning protocols. They provide the performance researchers need with ease of use as only common laboratory equipment is required and provide…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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MDR1/BCRP Double Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 and ABCG2 (MDR1/BCRP) efflux transporters. Synonyms: C2BBe1…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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MISSION(R) Lenti microRNA Inhibitor, Mouse mmu-miR-122-5p
MilliporeSigmaIndividual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a…
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MISSION(R) Lenti microRNA Inhibitor, Mouse mmu-miR-375-3p
MilliporeSigmaIndividual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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The Lenti CRISPR Human HPRT1 Positive Control Transduction Particles (LV04 vector) product is a critical positive control to monitor transduction efficiency for lentiCRISPRs. This control is produced from the sequence-verified CRISPR lentiviral plasmid targeting human HPRT1. The protein encoded by…
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BCRP/MRP2 Double Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The BCRP and MRP2 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional double knockout of the ABCG2 and ABCC2 (BCRP/MRP2) efflux transporters. Synonyms: …
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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MISSION(R) Lenti microRNA, Human hsa-miR-342-5p
MilliporeSigmaSigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the…
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BCRP Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCG2 (BCRP) efflux transporter. Synonyms: C2BBe1 Cells BCRP (-/-/-/-) …
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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MISSION(R) TRC3 ORF BFP Lentivirus High Titer Control
MilliporeSigmaThe MISSION TRC3 ORF BFP Lentivirus High Titer Control Transduction Particles contain an ORF insert expressing BFP, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome…