Chemicals, 7.4 ± 0.15
-
Phosphate Buffered Saline-Tween (20X, with 1% Tween-20, pH 7.4)
Boston BioProductsComponents: Sodium Phosphate Dibasic(202 mM), Potassium Phosphate Monobasic(35.2 mM), Potassium Chloride(54 mM), Sodium Chloride(2.74 M), Tween-20(1%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ±…
-
Sodium Phosphate Buffer (0.2 M, pH 7.4)
Boston BioProductsComponents: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(0.2 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.
-
Phosphate Buffered Saline-Tween (20X, with 2% Tween-20, pH 7.4)
Boston BioProductsComponents: Sodium Phosphate Dibasic(202 mM), Potassium Phosphate Monobasic(35.2 mM), Potassium Chloride(54 mM), Sodium Chloride(2.74 M), Tween-20(2%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ±…
-
Phosphate Buffered Saline (10X, for Western Blot Washing)
Boston BioProductsComponents: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(100 mM), Sodium Chloride(1.5 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
Tris-Buffered Saline (10X, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
RIPA Buffer (1X, with Triton X)
Boston BioProductsComponents: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Triton X-100(1%), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
-
HEPES Buffer (0.5 M, pH 7.4, pH Adjusted with KOH)
Boston BioProductsComponents: HEPES(500 mM), Potassium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
-
Tris-EDTA Buffer (pH 7.4, Autoclaved)
Boston BioProductsComponents: EDTA(~0.74%), Tris Base / Tris-HCl(~3.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle and/or filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.
-
Tris-Buffered Saline (20X)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Potassium Chloride(54 mM), Sodium Chloride(2.74 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
Phosphate Buffered Saline (10X, pH 7.4)
Boston BioProductsComponents: Sodium Phosphate Dibasic(101.4 mM), Potassium Phosphate Monobasic(17.6 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
Phosphate Buffered Saline-Tween (10X, with 1% Tween-20, pH 7.4)
Boston BioProductsComponents: Sodium Phosphate Dibasic(100 mM), Potassium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Tween-20(1%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15…
-
Tris Buffered Saline-Tween (20X, with 2% Tween-20, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(1 M), Sodium Chloride(3 M), Tween-20(2%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
Phosphate Buffered Saline (10X, pH 7.4, Low Endotoxin)
Boston BioProductsComponents: Sodium Phosphate Dibasic(100 mM), Sodium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: 2-8°C
-
Phosphate Buffered Saline (20X)
Boston BioProductsComponents: Sodium Phosphate Dibasic(202.8 mM), Potassium Phosphate Monobasic(35.2 mM), Potassium Chloride(54 mM), Sodium Chloride(2.74 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
Tris Buffered Saline-Tween (10X, with 0.5% Tween-20, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M), Tween-20(0.5 %) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
HEPES Buffer (1 M, pH 7.4)
Boston BioProductsComponents: HEPES(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
-
RIPA Buffer (with EDTA)
Boston BioProductsComponents: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), NP-40(1%), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), EDTA(5 mM) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
-
Tris Buffered Saline-Tween (20X, with 1% Tween-20, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(1 M), Sodium Chloride(3 M), Tween-20(1%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
Tris-Buffered Saline (10X, for Western Blot Washing)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
RIPA Buffer
Boston BioProductsComponents: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), Nonidet P-40 Substitute(1%) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
-
RIPA Buffer (2X)
Boston BioProductsComponents: Tris Base / Tris-HCl(100 mM), Sodium Chloride(300 mM), Nonidet P-40 Substitute(2%), Sodium Deoxycholate(1%), Sodium Dodecyl Sulfate (SDS)(0.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) …
-
Tyrode's Solution (with HEPES & Calcium)
Boston BioProductsComponents: Sodium Chloride(140 mM), Potassium Chloride(5 mM), HEPES(25 mM), Calcium Chloride Dihydrate(2 mM), Magnesium Chloride Hexahydrate(2 mM), Dextrose / Glucose(10 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4…
-
Sodium Phosphate Buffer (0.5 M, pH 7.4)
Boston BioProductsComponents: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(0.5M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.
-
Tris-Buffered Saline (10X, pH 7.4, Low Endotoxin)
Boston BioProductsComponents: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
Tris-Buffered Saline (20X, for Western Blot Washing, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(1M), Sodium Chloride(3 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
Tris Buffered Saline-Tween (10X, with 1% Tween-20, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M), Tween-20(1%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
HEPES Buffer (1 M, pH 7.4, pH Adjusted with KOH)
Boston BioProductsComponents: HEPES(1 M), Potassium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
-
RIPA Buffer (with EDTA & EGTA)
Boston BioProductsComponents: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), EDTA(5 mM), EGTA(1 mM), NP-40(1%), Sodium Dodecyl Sulfate (SDS)(0.1%), Sodium Deoxycholate(0.5%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 …
-
Potassium Phosphate Buffer (0.2 M, pH 7.4)
Boston BioProductsComponents: Potassium Phosphate Monobasic / Potassium Phosphate Dibasic(200 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.
-
Tyrode's Solution (with Bicarbonate)
Boston BioProductsComponents: Sodium Chloride(127 mM), Potassium Chloride(5 mM), Sodium Phosphate Monobasic(1.8 mM), Magnesium Chloride Hexahydrate(1 mM), Calcium Chloride Dihydrate(1 mM), Sodium Bicarbonate(25 mM), Dextrose / Glucose(5.5 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and…
-
Tris Buffered Saline-Wash Buffer (10X, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
-
MOPS Buffer (1 M, pH 7.4)
Boston BioProductsComponents: MOPS(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C