2,6-dichlorophenolindophenol (DCPIP) is a blue chemical compound used as a redox dye. In Oxidized form DCPIP is blue; whereas in reduced form DCPIP is colorless. The rate of photosynthesis can be measured by the rate at which this dye changes color (is reduced) when exposed to light in a…

Calcein is an indicator for the determination of calcium by titration with EDTA. The excitation wavelength and emission wavelength are 495/515 nm. It is suitable for fluorometric determination of calcium. It does not fluoresce in strong alkaline solution, but an intense fluorescence appears in the…

Brilliant Sulfaflavine is not the same as FIAT (Brilliant Sulfoflavin FF; Acid Yellow 7) which has only one methyl group para to the nitrogen. Brilliant sulfaflavine was used as fluorescent tracers to study the application rate of foliar fungicides when applied to soybeans for the control of rust.

Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. Applications Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and…

Ponceau S is used as protein stain for electrophoresis.

Lucifer Yellow CH is the hydrazine (-NH-CO-NH-NH2) form of the dye compared to Lucifer Yellow VS, which is the vinyl sulfone (-Ph-SO2-CH=CH2) form. It is a highly fluorescent dye useful in marking nerve cells. Lucifer yellow CH has been used to demonstrate electrical coupling between hamster…

Ponceau S is used as protein stain for electrophoresis.

Azomethine H is a reagent for the determination of boron in soil, plants, composts, manure, water and nutrient solutions.

Sulforhodamine B is a fluorescent dye. Sulforhodamine B can be used as a protein tag - by sulfamido condensation with a proteinaceous substrate containing a primary amine function. Sulforhodamine B will fluoresce red. Because of its red fluorescence it can be used in conjuction with fluorescein…

Acriflavine Hydrochloride is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine (approximately an 9 to 1 mixture respectively). Acriflavine Hydrochloride acts as an intercalating dye and also reported to inhibit mitochondriogenesis. It is an antiseptic and has been…

A red, anionic xanthene dye, has been used as a contrast or counter stain to hematoxylin, crystal violet and other nuclear stains, in both plant and animal tissue. Erythrosin B sodium salt is used as a counter stain to hematoxylin, crystal violet and other nuclear stains, in both plant and…

Primuline is a dye containing the benzothiazole ring system. The primulines are considered derivatives of dehydrothiotoluidine (aminobenzenyltoluylmercaptan). It is pale yellow in solution under visible light and is blue fluorescent under ultraviolet. Primulin is used in retrograde double…

Fluorescamine is a non-fluorescent reagent that reacts readily with primary amino groups in amino acids and peptides to form highly fluorescent derivatives, whereas the reagent and its degradation products are non-fluorescent. No significant fluorescence with ammonia. Low background due to…

p-Nitro Blue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the…

Hematoxylin is Gill’s Formulation 2; a nuclear counter stain of blue-violet color. It is extracted from the heartwood of the logwood tree. When oxidized it forms haematein, a compound that forms strongly coloured complexes with certain metal ions, the most notable ones being Fe(III) and…