Reagents, P, LGC, Biosearch Technologies, Chemicals
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OverExpress C41 (DE3) Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins…
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QuickExtract DNA Extraction Solution
LGC, Biosearch TechnologiesThe QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.
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E. cloni 10G Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of…
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Hybridase Thermostable RNase H 500 U
LGC, Biosearch TechnologiesHybridase™ Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA.
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FailSafe PCR System
LGC, Biosearch TechnologiesThe FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time….We promise!
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TG1 Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library…
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MasterPure DNA Purification Kit
LGC, Biosearch TechnologiesThe MasterPure™ DNA Purification Kit for Blood Version II can be used to isolate exceptionally pure genomic DNA from whole blood or buffy coat.
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Bst DNA Polymerase
LGC, Biosearch TechnologiesBst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5’-3’ polymerase activity and strand displacement activity, but it lacks 3’-5’ exonuclease activity. It also has reverse transcription…
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CircLigase II ssDNA Ligase
LGC, Biosearch TechnologiesCircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group
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Ampligase DNA Ligase
LGC, Biosearch TechnologiesAmpligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures
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LavaLAMP DNA Component Kits
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification…
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MasterPure Gram Positive DNA Purification Kit 100 Purif.
LGC, Biosearch TechnologiesThe MasterPure™ Gram-Positive DNA Purification Kit provides all of the reagents needed to purify DNA from Gram-positive bacteria.
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Riboguard Rnase Inhibitor
LGC, Biosearch TechnologiesRiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.
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Sbeadex viral RNA Purification Kit
LGC, Biosearch Technologiessbeadex viral RNA purification kits are magnetic-bead based kits are validated for research use only using AccuPlex™ reference material (SeraCare), replicating a real-life clinical sample as far as practically possible, and are compatible with Biosearch Technologies’ COVID-19 detection kit.…
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MasterPure Complete DNA & RNA Purification Kits
LGC, Biosearch TechnologiesThe MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
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Recovery Medium
LGC, Biosearch TechnologiesThe LGC recovery medium is used in chemical laboratories and helps in the growth and fostering off competent cells thus allowing the highest possible transformation efficiency.
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RNase I 1,000 U
LGC, Biosearch TechnologiesRNase I degrades single-stranded RNA to nucleoside 3´ monophosphates via 2´,3´ cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3 unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C…
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NxSeq UltraLow DNA Library Kits
LGC, Biosearch TechnologiesThe NxSeq UltraLow DNA Library Kit is used to build DNA fragment libraries for Illumina sequencers. It requires only 50 pg to 75 ng of input DNA. This 96 rxn kit is optimized for library prep in 96-well plates and requires a NxSeq HT Dual Indexing Kit to complete library construction.
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E. cloni EXPRESS BL21(DE3) Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of…
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HI-Control BL21(DE3) Chem Comp Cells 12 rxns (SOLOs)
LGC, Biosearch TechnologiesThe HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.
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LavaLAMP DNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer…
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Ready-Lyse Lysozyme Solution
LGC, Biosearch TechnologiesReady-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.
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ClearColi BL21(DE3) Electrocompetent Cells
LGC, Biosearch TechnologiesClearColi® BL21(DE3) cells are the first commercially available competent cells with a modified LPS that does not trigger the endotoxic response in mammalian cells. ClearColi™ cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by…
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E. cloni 5-alpha Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is…
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MC1061 F- Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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RapiDxFire Thermostable Reverse Transcriptase
LGC, Biosearch TechnologiesRapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10…
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RapiDxFire qPCR 5X Master Mix GF
LGC, Biosearch TechnologiesRapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…
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Episcript Rnase H- Reverse Transcriptase
LGC, Biosearch TechnologiesEpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity
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Proteinase K 2 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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EZ-TN5 TRANSPOSASE 10 U
LGC, Biosearch TechnologiesEZ-Tn5™ Transposase is a hyperactive Tn5 transposase for random insertion of an EZ-Tn5 Transposon into any cloned DNA in vitro or for preparing an EZ-Tn5 Transposome for random insertion of a EZ-Tn5 Transposon into the genomic DNA of living bacteria.
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MMLV REVERSE TRANS 1ST-STRD CDNA SYN KIT 50 rxns
LGC, Biosearch TechnologiesThe MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit is optimized for generating full-length first-strand cDNA from total RNA preparations or purified Poly(A)+ RNA.
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Green Fluorescent Dye 500 rxns
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.