LGC, Biosearch Technologies Chemicals Reagents P
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ClearColi BL21(DE3) Electrocompetent Cells
LGC, Biosearch TechnologiesClearColi® BL21(DE3) cells are the first commercially available competent cells with a modified LPS that does not trigger the endotoxic response in mammalian cells. ClearColi™ cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by…
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QuickExtract RNA Extraction Kit 50 ml
LGC, Biosearch TechnologiesThe QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis.
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COPYCUTTER INDUCTION SOLUTION 25 ml
LGC, Biosearch TechnologiesPrepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.
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Tissue & Cell Lysis Solution 600 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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CircLigase ssDNA Ligase
LGC, Biosearch TechnologiesCircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group
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Ampligase DNA Ligase
LGC, Biosearch TechnologiesAmpligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures
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TRANSFORMAX EPI300-T1R ELECTRO E. COLI 20 rxns
LGC, Biosearch TechnologiesPhage T1-resistant electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.
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LavaLAMP DNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer…
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RapiDxFire qPCR 5X Master Mix GF
LGC, Biosearch TechnologiesRapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…
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MasterPure Complete DNA & RNA Purification Kits
LGC, Biosearch TechnologiesThe MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
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Red Cell Lysis Solution 1200 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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E. cloni 10G CLASSIC Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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Transformax Epi300 Electrocomp E. Coli
LGC, Biosearch TechnologiesElectrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.
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TE Buffer 70 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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NxGen RNAse Inhibitor
LGC, Biosearch TechnologiesRNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.
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Hybridase Thermostable RNase H 500 U
LGC, Biosearch TechnologiesHybridase™ Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA.
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RNase-Free DNase I
LGC, Biosearch TechnologiesRNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation.
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Exonuclease VII 250 U @ 10 U/µl
LGC, Biosearch TechnologiesExonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´′→3´ and 3´′→5´ exonuclease activities.
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Exo-Minus Klenow DNA Polymerase 1,000 U
LGC, Biosearch TechnologiesExo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´′→3´ and 3´′→5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A).
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TRANSFORMAX EPI300 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically competent E. coli engineered to regulate the copy number of CopyControl Fosmid clones.
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Riboguard Rnase Inhibitor
LGC, Biosearch TechnologiesRiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.
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NxSeq Single Indexing Kits
LGC, Biosearch TechnologiesEach NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reaction for all primer sets). Set A contains Primer Indexing…
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TG1 Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library…
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BAC-Opt Replicator v2.0 Electrocomp Cells
LGC, Biosearch TechnologiesBAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 10^10 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells…
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SS320 (MC1061 F) Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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MC1061 F- Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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NxGen T4 DNA Ligase
LGC, Biosearch TechnologiesT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
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Green Fluorescent Dye 500 rxns
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.
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CircLigase II ssDNA Ligase
LGC, Biosearch TechnologiesCircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group
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Poly(A) Polymerase Tailing Kit 50 Rxns
LGC, Biosearch TechnologiesThe Poly(A) Polymerase Tailing Kit provides the enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3´ end of any RNA. Poly(A) Polymerase is encoded by an E. coli gene that has been cloned into a plasmid and overexpressed in an E. coli strain.
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TRANSFORMAX EC100 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically Competent E. coli cells are ideal for most cloning applications. The cells provide very high transformation efficiency.