Reagents, P, Reagents, LGC, Biosearch Technologies, PCR Reagents
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Riboguard Rnase Inhibitor
LGC, Biosearch TechnologiesRiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.
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Tissue & Cell Lysis Solution 600 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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E. cloni 10G CLASSIC Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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E. cloni 10G ELITE Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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Ready-Lyse Lysozyme Solution
LGC, Biosearch TechnologiesReady-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.
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Plasmid-Safe ATP-Dependent DNase
LGC, Biosearch TechnologiesPlasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
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Endura Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…
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MasterPure Complete DNA & RNA Purification Kits
LGC, Biosearch TechnologiesThe MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
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LavaLAMP DNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer…
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MasterPure Yeast RNA Purification Kits
LGC, Biosearch TechnologiesThe MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi.
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LavaLAMP DNA Component Kits
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification…
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LavaLAMP™ RNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which…
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Durascribe T7 Transcription Kits
LGC, Biosearch TechnologiesThe DuraScribe® T7 Transcription Kit produces 2'-Fluorine-modified RNA transcripts—called DuraScript® RNA—that are completely resistant to RNase A. Ideal for SELEX RNA-aptamer selection procedures.
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Bst DNA Polymerase
LGC, Biosearch TechnologiesBst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5’-3’ polymerase activity and strand displacement activity, but it lacks 3’-5’ exonuclease activity. It also has reverse transcription…
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MC1061 F- Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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RapiDxFire Thermostable Reverse Transcriptase
LGC, Biosearch TechnologiesRapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10…
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Copycutter Epi400
LGC, Biosearch TechnologiesCopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins
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Exonuclease III 25,000 U @ 200 U/µl
LGC, Biosearch TechnologiesExonuclease III digests duplex DNA in a 3´′→5´ direction from a nick, a blunt end, or 3´-recessed end, producing stretches of ssDNA on the opposite strand
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Ampligase 10X Reaction Buffer 5 ml
LGC, Biosearch TechnologiesAmpligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures
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RapiDxFire qPCR 5X Master Mix GF
LGC, Biosearch TechnologiesRapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…
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E. cloni 10G BAC-Optimized Electrocomp Cell 25 rxns
LGC, Biosearch TechnologiesE. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 10^7 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning. Manufactured using a novel procedure that maximizes transformation efficiencies with very large…
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RNase-Free DNase I
LGC, Biosearch TechnologiesRNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation.
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E. cloni 5-alpha Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is…
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ClearColi BL21(DE3) Electrocompetent Cells
LGC, Biosearch TechnologiesClearColi® BL21(DE3) cells are the first commercially available competent cells with a modified LPS that does not trigger the endotoxic response in mammalian cells. ClearColi™ cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by…
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MasterAmp 10X PCR Enhancer 10 ml
LGC, Biosearch TechnologiesFor use with the MasterAmp™ PCR Optimization Kits.
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NxSeq Single Indexing Kits
LGC, Biosearch TechnologiesEach NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reaction for all primer sets). Set A contains Primer Indexing…
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HI-Control 10G Chemically Competent Cells 12 rxns (SOLOs)
LGC, Biosearch TechnologiesThe HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.
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EZ-TN5 TRANSPOSASE 10 U
LGC, Biosearch TechnologiesEZ-Tn5™ Transposase is a hyperactive Tn5 transposase for random insertion of an EZ-Tn5 Transposon into any cloned DNA in vitro or for preparing an EZ-Tn5 Transposome for random insertion of a EZ-Tn5 Transposon into the genomic DNA of living bacteria.
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TRANSFORMAX EC100 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically Competent E. coli cells are ideal for most cloning applications. The cells provide very high transformation efficiency.
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Uracil N-Glycosylase 1,000 U
LGC, Biosearch TechnologiesUracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate
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OmniCleave Endonuclease 50,000 U
LGC, Biosearch TechnologiesOmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides.