Nucleases for PCR

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Nucleases for PCR

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  • RNase Block

    Agilent Genomics

    A 50-kDa protein that specifically inhibits common eukaryotic RNases Only 25 to 40 U is required for a standard transcription reaction. Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts. …

  • DNase I, RNase Free

    Agilent Genomics

    DNase I, Rnase Free enzyme from bovine pancreas produces no noticeable RNA degradation. Produces no noticeable RNA degradation One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25-µL reaction volume. Incubation of 100-fold molar excess DNase I…

  • RNace-IT Ribonuclease Cocktail Free of contaminating DNase activity. Active in any neutral buffer (pH 6.0-9.0) or water with sodium chloride concentrations ? 100 mM. 20 units will degrade the RNA in a 1.5 ml plasmid preparation. RNace-IT ribonuclease cocktail is a mixture of two…

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