DNase I, Rnase Free enzyme from bovine pancreas produces no noticeable RNA degradation.
Produces no noticeable RNA degradation
One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25-µL reaction volume.
Incubation of 100-fold molar excess DNase I with RNA for one hour at 37°C produces no noticeable RNA degradation upon analysis by denaturing polyacrylamide gel electrophoresis.
Reaction conditions: 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37°C.;Storage Buffer: 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50 % glycerol (v/v). (Dilutions should be made in this buffer.)
DNase I, Rnase Free from bovine pancreas catalyzes the degradation of double-stranded DNA into oligonucleotides 1,2 and mononucleotides. This enzyme has been isolated as a mixture of four isoenzymes from bovine pancreas that cut preferentially next to pyrimidine nucleotides.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.