10 L
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Molecular Biology Grade Water
Quality BiologicalMolecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes, to yield a product with an endotoxin level of less than 0.03 EU/mL. DNase, RNase and protease tested Endotoxin…
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Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagles medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate.…
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Basal Medium Eagle (BME) is one of the most commonly used of all cell culture media. This medium is the result of numerous studies from the late 1950's analyzing the nutrient requirements and other components essential for the growth of cells in culture. Traditionally, BME has been used in studies…
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TNM-FH medium is a modification of Grace's insect medium. The modification was developed by Dr. W. F. Hink to grow Cabbage looper cell line TN-368. Presently, this medium is used to grow cells derived from a variety of lepidopterans species. IM008 is supplemented with lactalbumin hydrolysate and…
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Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely…
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Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell…
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Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. …
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Schneider's Insect Medium
HiMediaSchneider's insect medium has been specially formulated for the in vitro culture of Drosophila melanogaster cells and tissues. A number of cell lines derived from Drosophila melanogaster are now available and are extensively used in genetic and molecular biology research. Drosophila cells are also…
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IPL-41 Insect Medium
HiMediaIPL-41 is a modification of Goodwin's original IPL formulation. It was developed by Weiss et al of United States Department of Agriculture (USDA) Insect Pathology Laboratory for large scale propagation of a cell line derived from fall armyworm, Spodoptera frugiperda. Weiss supplemented the basal…
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Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric…
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D-22 Insect Medium
HiMediaD-22 medium (1976) is the final modification of the original D20 medium developed by Echalier and Ohanessian (1970). The medium composition closely initiates the principal characteristics of the body fluid of Drosophila 3rd instar larvae. This medium has been extensively used to grow many…
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Basal Medium Eagle (BME)
HiMediaBasal Medium Eagle developed by Harry Eagle is a combination of essential nutrients in appropriate concentrations for monolayer cultivation of a wide variety of normal and transformed cells. The medium was initially developed as a result of studies to determine the nutritional requirements of HeLa…
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Sheilds and Sang M3 medium is based on the original formula of D22 medium. The medium supports the growth of the cells derived from Drosophila melanogaster. Sheilds and Sang (1977) eliminated chloride salts present in the previous formulation and introduced potassium and sodium ions in the form of…
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RPMI 1640
HiMediaRoswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support…
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Grace's Insect Medium
HiMediaGrace's medium is a modification of the original formula developed by Wyatt. Grace (1962) improved the medium by introducing ten water soluble vitamins in Wyatt's formula. Grace successfully established four strains of cells from ovarian tissue of Australian emperor gum moth using this medium.…
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Mitsuhashi and Maramorosch Insect Medium is specially used for the growth and propogation of mosquito cell lines especially Aedes aegypticus . It was originally developed to grow cells derived from Leafhopper. Subsequently, it has been used to culture cells derived from a number of insect species.…
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TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV…
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Grace's Suppl Media (Hink's)
bioWORLDGrace's Insect Cell Culture Media is a modification of Wyatt's medium and was originally formulated to support the growth of cells derived from the Australian emperor gum moth, Anthere eucalypti . When supplemented with the proper components, this medium has been shown to support a variety…