LB Agar Plates are used for the growth and maintenance of E. coli strains used in molecular microbiology procedures. Applications The following variations of LB Agar Plates are nutritionally rich media designed for growth of pure cultures of recombinant strains. LB Agar plates are suitable…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…

LB Agar 1.5% Lennox provides all nutritional requirements of these organisms. LB Agar 1.5% Lennox contains half the sodium chloride level of the Miller formulation of LB Agar. Just autoclave to melt and pour. Applications LB Agar 1.5% Lennox is nutritionally rich. It was developed by…

This modification of Dulbecco's MEM was developed by Dr. Iscove and his coworkers to assist in their studies of hematopoietic stem cells. They modified Dulbecco's MEM by the addition of HEPES buffer, selenium, additional amino acids, vitamins and sodium pyruvate, and potassium nitrate…

Sodium pyruvate is commonly utilized as an additive in culture media and broth as a carbon source, similar to glucose, as pyruvate is the end product of the glycolysis pathway. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…

EMEM (Eagle’s Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without L-Glutamine Applications EMEM has a higher amino acid concentration and 2 to 5 times greater…

Super Broth is used for the mass cultivation of Escherichia coli for high yield protein and plasmid DNA production. Has 220% more peptone and 300% more yeast extract than LB Autoclave sterilized Applications Super Broth is the best commonly used E. Coli growth medium for plasmid…

Nutritionally rich media designed for growth of pure cultures of recombinant strains. 10 g/L of salt formulation Autoclave sterilized LB Agar, Miller and LB Broth, Miller are based on LB Medium as described by Miller for the growth and maintenance of E. coli strains used in molecular…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES MEM Spinner was developed when calcium component was removed from the MEM medium, and resulted in a medium that supports the…

Compared to other basal media, Ham’s F-12 contains a wider variety of components, and no proteins or growth factors. Without hypoxanthine and L-glutamine Ham’s F-12 uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH. …

LB Broth, Lennox is a ready-to-use microbial growth medium used for the cultivation of E. coli. Low (5 g/L) salt formulation Autoclave sterilized Applications LB Broth is a low-salt formulation in comparison to its counterpart LB Broth, Bertani Miller. Lennox is also nutrient rich…

DMEM is one of the most widely used modifications of Basal Medium Eagle (BME). DMEM was originally developed as a richer version of BME; with 4 times higher concentrations of amino acids, vitamins and some additional components than BME. DMEM is commonly used for the culture of normal and…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations EMEM Enriched is regular EMEM supplemented with 2X penicillin, streptomycin and L-glutamine.

RPMI was developed to support lymphoblastoid cells in culture, RPMI-1640 has now been successfully used for the culture of a wide range of anchorage independent cells. Although intended for use with serum supplementation, RPMI-1640 has been shown to support the serum-free growth of a number of…

QBI's Serum Free Cryo Medium is a sterile, 2X concentrated freezing medium containing DMSO  developed for the cryopreservation of cells grown under serum free conditions. Serum Free Cryo Medium is an animal-free, protein-free, chemically defined medium containing DMSO and other…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

Applications The FBS in the Cryopreservation Medium improves the post-freezing recovery and survival after cryopreservation, while DMSO preserves the cells during freeze. Cryopreservation Medium with Fetal Bovine Serum (FBS) and Calf Serum is a complete cryopreservation medium with 10% DMSO.

Hanks' Balanced Salt Solution (HBSS) provides a buffering system to maintain the physiological pH range and osmotic balance of the culture media. HBSS provides cells with a source of water and essential inorganic ions, and carbohydrate as an energy source. The HBSS without Ca and Mg…

M9 CA Media contains the essentials for bacterial species to grow. Glucose as a carbon source and casamino acids as a nitrogen source. Important components of M9 CA medium are the carbon source from glucose, a nitrogen source from ammonia chloride, and electrolytes from sodium, chloride,…

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators…

LB Agar Plates are used for the growth and maintenance of E. coli strains used in molecular microbiology procedures. Without any antibiotics Applications The following variations of LB Agar Plates are nutritionally rich media designed for growth of pure cultures of recombinant strains.…

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications for a range of cell culture applications. Applications DMEM is one of the most widely used modifications of Basal Medium Eagle…

Dr. Williams and his coworkers’ studies on hepatocytes in culture led to the development of Williams’ Medium E. Without L-glutamine Applications This rich medium was used to culture the hepatocytes derived from an enrichment procedure also developed by Dr. Williams and his colleagues in…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate Without HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

QBSF-60 is a proprietary serum-free medium that supports the expansion of human hematopoietic stem/progenitor cells. QBSF-60 supports CD34+ cells derived from normal bone marrow, cord blood, peripheral blood stem cells mobilized with cytokines for ex-vivo expansion and differentiation to…

YES Agar Plates are for use of propagation and maintenance of yeasts for use in molecular biology Applications YES Agar Plates are for the growth of yeast.

Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…

YPD/YPED Plates are used for the growth of Saccharomyces cerevisiae (yeast). Applications A solid medium for the propagation of yeast.

YPD is a media variant derived from YPAD. YPAD consists of nutrient base (yeast extract and peptone), adenine supplement and a carbon source. YPD’s formulation is the same but without the additional adenine. YPD 1/2 Plates are useful for subculturing of untransformed yeast, yeast two-hybrid…

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes, to yield a product with an endotoxin level of less than 0.03 EU/mL. DNase, RNase and protease tested Endotoxin…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L Sodium Bicarbonate Without HEPES buffer Applications E199 has broad species applicability, particularly for cultivation of…