McCoy's 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker 256 carcinoma cells along with a wide range of human and rat normal and transformed cells. 

  Technical Data Sheet

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of…

Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging.  Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action.  EDTA sequesters these ions and thus enhances the efficacy of…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES MEM Spinner was developed when calcium component was removed from the MEM medium, and resulted in a medium that supports the…

Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.  This research indicated that additions made to BME aided cell propagation.  MEM is modified with…

Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates obtained from autolyzed yeast. This is the water soluble portion with intact B-complex vitamins and can be used as an additive for culture media. 10,000 MW cut-off

Sodium bicarbonate Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment.  Bicarbonate is a weak buffer with that makes maintaining a pH range of 7.2 - 7.6 somewhat difficult, but it is non-toxic, and is a…

Sodium bicarbonate Solution 7.5%. Used as a biological buffer in cell culture media to maintain physiological pH.

Corning's line of specialty media is optimized for those who require media for a wide range of specialized applications and cell lines. The use of T-lymphocytes in adaptive immunotherapy is emerging as an important tool in the treatment of cancer and other clinical applications.…

Prepared RODAC™ (Replicate Organism Detection and Counting) plates are recommended for the detection and enumeration of microorganisms present on surfaces of sanitary importance.

Corning BioCoat Gelatin cultureware provides an attachment and growth promoting substrate for the culture of a variety of cell types. Gelatin is commonly used in the culture of vascular endothelial cells, muscle, embryonic stem (ES) cells, and F9 teratocarcinoma cells. It is also suitable for…

10x PBS Solution (Phosphate Buffered Saline): This PBS formulation is recommended for use in immunohistology protocols. Each package contains 4 L of 10 concentrated PBS or 40 liters of 1 x solution. Each 1 x PBS solution contains 137 mM Sodium Chloride, 2.7mM Potassium Chloride and 10mM…

Thomas Nos. B003K38 and B003K39 Iscove's Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density.  A modified form of Dulbecco's Modified Eagle Medium (DMEM), Iscove's contains additional amino acids,…

Crosstex Prepared Culture Media consists of an exclusively formulated Trypticase® Soy Broth (TSB) modified with pH indicator. A reduced incubation time of 24 hours for steam and hydrogen peroxide (VH2O2) sterilization has been validated when Crosstex Biological Indicator Spore Strips and Discs…

Uridine is a ribonucleoside that has Uracil attached to its ribose ring. It is an important component of carbohydrate metabolism and is essential for both RNA and DNA synthesis. The monophosphate form of uridine (UMP) is formed from orotidylic acid (orotidine 5'- monophosphate) in a decarboxylation…

The 30° angled cassette features an extra-large writing surface and two side panels ideal for labeling. The cassette has an integral lid that snaps apart and securely locks into the base of the cassette. The lid can be removed and reattached repeatedly to access the specimen. Note: These cassettes…

Corning transfectagro Reduced Serum Medium is designed to maximize the growth of a variety of cell types under reduced serum conditions, generally allowing for at least a 50% reduction in serum use with minimal adaptation. transfectagro has also been proven to improve transient transfection…

Poly-D-Lysine, used to culture a wide variety of cell types, particularly neurons, glial cells, and transfected cells. (20 mg) Source: Synthetic Quality: Shown to promote cell attachment and spreading of rat cerebellar granule cells Filtered (0.2 µm membrane) and tested and found negative…

All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…

Specialized for use in insect cell culture Poloxamer 188 is a surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and thawing, and stirring. It is a non-ionic detergent with an average molecular weight of 8400Da consisting of a central…

Applications Standard, unstabilized solutions of L-glutamine spontaneously break down over time to release cytotoxic amines. Glutamine XL is a stabilized solution that prevents degradation and cytotoxicity, with the benefit of a long shelf life. The incorporated L-alanine protects the unstable…

A modification of Ham's F-12, F-12K contains higher concentrations of amino acids, sodium pyruvate and other components than the original. It is formulated to support the growth of cells including differentiated rat and chicken cell types and primary human hepatocytes. This medium contains…

Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the…

EMEM (Eagle’s Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without L-Glutamine Applications EMEM has a higher amino acid concentration and 2 to 5 times greater…

This Tryptic Soy Broth (TSB) product is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. The product is a…

Dulbecco's Phosphate Buffered Saline is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a…

1× Park and Terasaki's Modification Of McCoy 5A is used for the primary culture of cells from a wide range of tissues. Recommend for production of virus in primary cell cultures. It is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L Sodium Bicarbonate Without HEPES buffer Applications E199 has broad species applicability, particularly for cultivation of…

Corning ECM mimetic surfaces and substrates contain biologically active, synthetic, animal-free peptides that have been rationally designed to mimic the cell attachment motifs of native ECM proteins. The peptides enable optimal cell binding and signaling in a broad range of serum-free,…

Laminin (LM), a major component of basement membranes, is a multifunctional glycoprotein that is used as a substrate to culture and maintain differentiated function of a wide variety of cells. Laminin has been shown in culture to stimulate neurite outgrowth, promote cell attachment, chemotaxis,…

For some applications, the use of a combination of ECM proteins, such as Laminin (LM) and Fibronectin (HFN) or LM and attachment factors such as Poly-D-Lysine (PDL) or Poly-L-Orinthine (PLO) has been shown superior to the use of either alone. Corning BioCoat PDL/LM and PLO/LM Cultureware is…