Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham's Nutrient Mixtures F12 was…

TNM-FH medium is a modification of Grace's insect medium. The modification was developed by Dr. W. F. Hink to grow Cabbage looper cell line TN-368. Presently, this medium is used to grow cells derived from a variety of lepidopterans species. IM008 is supplemented with lactalbumin hydrolysate and…

IPL-41 is a modification of Goodwin's original IPL formulation. It was developed by Weiss et al of United States Department of Agriculture (USDA) Insect Pathology Laboratory for large scale propagation of a cell line derived from fall armyworm, Spodoptera frugiperda. Weiss supplemented the basal…

Schneider's insect medium has been specially formulated for the in vitro culture of Drosophila melanogaster cells and tissues. A number of cell lines derived from Drosophila melanogaster are now available and are extensively used in genetic and molecular biology research. Drosophila cells are also…

Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham's Nutrient Mixture F12 was originally…

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely…

D-22 medium (1976) is the final modification of the original D20 medium developed by Echalier and Ohanessian (1970). The medium composition closely initiates the principal characteristics of the body fluid of Drosophila 3rd instar larvae. This medium has been extensively used to grow many…

Sheilds and Sang M3 medium is based on the original formula of D22 medium. The medium supports the growth of the cells derived from Drosophila melanogaster. Sheilds and Sang (1977) eliminated chloride salts present in the previous formulation and introduced potassium and sodium ions in the form of…

Grace's medium is a modification of the original formula developed by Wyatt. Grace (1962) improved the medium by introducing ten water soluble vitamins in Wyatt's formula. Grace successfully established four strains of cells from ovarian tissue of Australian emperor gum moth using this medium.…

Mitsuhashi and Maramorosch Insect Medium is specially used for the growth and propogation of mosquito cell lines especially Aedes aegypticus . It was originally developed to grow cells derived from Leafhopper. Subsequently, it has been used to culture cells derived from a number of insect species.…

TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV…