Pectinase is a macerating enzyme comprised of pectintranseliminase, polygalacturonase, pectinesterase, xylanase, hemicellulases and cellulases. Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase causes random hydrolysis of…

HRP is a plant glycohemoprotein that belongs to the ferroprotoporphyrin group of peroxidases. HRP is composed of seven isozymes. All isozymes contain photohemin IX as prosthetic group. Neutral and amino sugars account for approximately 18% of the enzyme. The iron-containing hemin group is…

β-Glucosidase is a glucosidase enzyme. It is an exocellulase with specificity for a variety of β-D-glycoside substrates. β-Glucosidase is used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries,…

Catalase occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. It promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.…

Pyruvate kinase from rabbit muscle is a tetramer consisting of four equal subunits of molecular weight 57 kDa. Pyruvate kinase catalyzes the following reaction: ADP + Phosphoenolpyruvate → Pyruvate + ATP . Both Mg2+ and K+ are required metal cofactors for optimal activity. The enzyme is…