Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) is a bifunctional enzyme that converts arachidonic acid to a hydroperoxy endoperoxide (PGG₂) and reduces the endoperoxide to the corresponding alcohol (PGH₂), the precursor of prostaglandins, thromboxanes, and…

Cayman’s Lipid Hydroperoxide Assay measures the hydroperoxides directly utilizing the redox reactions with ferrous ions. An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure…

Prostaglandin I (Prostaglandin I2; PGI2) is a potent vasodilator and inhibitor of platelet aggregation that is formed from arachidonic acid primarily in the vascular endothelium and renal cortex by sequential activities of cyclooxygenase (COX) and PGI2 synthase. It is non-enzymatically hydrated to…

17-phenyl trinor PGF2α is a metabolically stable analog of PGF2α and is a potent agonist for the FP receptor. The ethyl amide analog has been approved for use as an ocular hypotensive drug, sold under the Allergan trade name Bimatoprost. There is some evidence that unmetabolized…

FAAH is a cytosolic serine hydrolase responsible for the degradation of fatty acid amides, including AEA. Finding inhibitors to FAAH could offer a beneficial approach toward the treatment of pain, obesity, and various neurological diseases where higher endocannabinoid activity would be beneficial.…

LTB4 is synthesized from arachidonic acid by the combined action of 5-LO and LTA4 hydrolase. LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release,…

This assay offers the convenience of a fast assay (2 hour incubation; 1 hour development) while still achieving a detection limit (80% B/B0) of 10 pg/ml.

Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid via the 5-lipoxygenase (5-LO) pathway in leukocytes. LTC4, LTD4, and LTE4 are collectively referred to as cysteinyl leukotrienes (CysLTs). LTC4 and LTD4 are potent mediators of asthma and hypersensitivity.…

Cayman’s PGE2 Express ELISA is a competitive assay that permits the rapid measurement of PGE2 from biological samples, requiring only 1 hour incubation and development times for each step. The foundation of the assay is Cayman’s high-affinity, well-characterized PGE2 monoclonal, the…

The COX (human) Inhibitor Screening Assay Kit directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using a broadly specific antiserum that binds to all the major prostaglandin compounds. This assay includes…

Cayman’s sPLA2 Assay provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s with the exception of cPLA2. Upon hydrolysis of the thioester bond at the sn-2…

COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-1 (ovine) Inhibitor Screening Assay measures PGF2α that is produced by SnCl2 reduction of COX-1-derived PGH2.…

Maresins (macrophage mediators in resolving inflammation) are members of a class of potent anti-inflammatory and pro-resolving mediators that are synthesized by macrophages in the presence of DHA. MaR1 is produced via oxidation of endogenous DHA by 12-LO. MaR1 has been shown to reduce neutrophil…

PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy PG dehydrogenase and D13-reductase. Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α. The Cayman’s…