Chromatography Reagents

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Thermo Scientific Chromatography Reagents

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  • HMDS (Hexamethyldisilazane)

    Thermo Scientific

    HMDS (Hexamethyldisilazane) greatly extends the practical range of GC, improving chromatographic results in the silylation of sugars and related substances. Suitable for deactivating and coating chromatographic supports Monofunctional, making polymerization not possible and eliminating surface moisture

  • Silylation Grade Solvents

    Thermo Scientific

    Silylation Grade Solvents are manufactured to meet exacting silylation needs. Purified, dried and packaged under nitrogen in convenient 50mL Hypo-Vial Sample Storage Vials Supplied with elastomer septa, allowing immediate access to the sample without exposure to moisture and oxygen Use polar…

  • Use Thermo Scientific™ BSTFA + 1% TMCS Silylation Reagent for analytes that are difficult to silylate. Stronger than BSTFA alone, this catalyzed formulation is ideal for derivatizing compounds such as fatty acid amides and slightly hindered hydroxyls for GC analysis. Excellent for…


    Thermo Scientific

    Offers maximum volatility Trimethylsilyl donor strength comparable to BSA and BSTFA Reacts to replace labile hydrogens on a wide range of polar compounds with a Si(CH3)3 group Used to prepare volatile and thermally stable derivatives for GC and MS Volatile byproduct,…

  • Tri-Sil BP Reagent derivatizes alcohols, phenols, organic acids, aromatic amides and amines. Excellent for unhindered steroids, but not recommended for carbohydrates. Reacts with: Alcohols, phenols, some enols and other hydroxyl and polyhydroxyl compounds to form trimethylsilyl esters …

  • BSTFA Silylation Reagent

    Thermo Scientific

    Improve GC analysis with Thermo Scientific™ BSTFA Silylation Reagent. The highly volatile byproducts mono(trimethylsilyl)trifluoroacetamide (BSTFA) and trifluoroacetamide elute with the solvent front to ensure excellent chromatographic separations. This silylation reagent reacts with a wide…

  • TMSI (N-Trimethylsilylimidazole) is the strongest silylator available for carbohydrates and steroids. Reacts quickly and smoothly with hydroxyls and carboxylic acids but not with amines Especially useful in multiderivatization schemes for compounds containing both hydroxyl and amine groups …

  • Methoxamine (MOX) Reagent

    Thermo Scientific

    Methoxamine (MOX) Reagent is useful for preparing oximes of steroids and ketoacids prior to silylation. 2% methoxyamine·HCl (M.W. 83.51) in pyridine Prevents formation of multiple derivatives when enols are present during silylation Supplied in amber Thermo Scientific Hypo-Vial Sample Storage…

  • TBT (TMSI:BSA:TMCS) Reagent is a catalyzed silylation reagent formulation containing three parts TMSI, three parts BSA and two parts TMCS. Converts all classes of hydroxyl groups to TMS ethers Under usual conditions, the reaction is complete in a short period of time at 60 to 80°C, although…

  • TMCS (Trimethylchlorosilane)

    Thermo Scientific

    TMCS (Trimethylchlorosilane) is an excellent catalyst for difficult-to-silylate compounds. Excellent adjunct for forming trimethylsilyl ethers for GC determinations Used to prepare TMS derivatives of organic acids

  • For easy, colorimetric detection and measurement of primary amines Thermo Scientific TNBSA Solution is a 5% solution of trinitrobenzene sulfonic acid in methanol that reacts with primary amines (peptides or amino acids) to yield a soluble colored product, a property useful for various assay…

  • Tri-Sil TP Reagent derivatizes hydroxyl compounds, particularly carbohydrates. Silylates alcohols and phenols, organic acids, hydroxylamines, amino acids, carbohydrates, flavonoids, glycols and polyglycols, nucleotides, steroids, hydroxyl acids, barbituates, narcotics, indoles and vitamins Does…


    Thermo Scientific

    MTBSTFA and MTBSTFA+1% TBDMCS offer stable TBDMS (tert-butyldimethylsilyl) derivatization. Derivatizes hydroxyl, carboxyl, thiol and primary and secondary amines Typical yields are >96% Provides TBDMS ethers that are 104 times more stable to hydrolysis than TMS ethers Reaction byproducts…

  • Employ a reagent-catalyst solvent mixture, for one-step derivatization with the Thermo Scientific™ Tri-Sil HTP (HDMS:TMCS:Pyridine) Reagent, Composed of HMDS, TMCS and high-purity pyridine, it rapidly produces TMS derivatives of polar compounds for GC analysis and biochemical synthesis. This…

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