Chaotropic Agents
Chaotropic reagents are essential for use within the lab to aid in the denaturation of proteins by weakening the hydrophobic effect. The single-step technique separates RNA from DNA after extraction with an acidic solution. Chaotropic agents disrupt the structure and stability of biological molecules, including protein and nucleic acid. Protein modification reagents include urea, which is ideal for breaking hydrogen bone and disrupting the native structure of biomolecules. It also includes guanidine hydrochloride, which is perfect for disrupting interactions holding proteins together. Formamide is an agent used in molecular biology to denature nucleic acids and can make them more accessible for analysis. Chaotropic agents are precious for the study and manipulation of biomolecules. This is particularly useful in protein purification, nucleic acid extraction, and DNA sequencing, where the goal is to isolate or manipulate specific biomolecules.
Also available at Thomas Sci – DNA isolation & purification supplies and reagents for a variety of laboratory purposes.
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Guanidine Thiocyanate
G-BiosciencesFeatures Synonym: Guanidinium thiocyanate CAS#: 593-84-0 Molecular Formula: CH5N3 CHNS Molecular Weight: 118.16
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Guanidine Hydrochloride, Molecular Biology Reagent
MP BiomedicalsGuanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds, it is a strong chaotropic agent. Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a free flowing powder…
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Guanidine Hydrochloride
G-BiosciencesA selection of strong chemicals to aid in the denaturation of proteins. Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. The chaotropes also act on all proteins to unfold them and alter their three-dimensional structure. …
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Guanidine Hydrochloride, Ultra Pure
MP Biomedicals* Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins * Used in the isolation of RNA * Solubilizes insoluble or denatured proteins such as inclusion bodies * Can be used for the recovery of periplasmic proteins
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Urea, Ultra Pure
MP BiomedicalsUrea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle. Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with…
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Guanidine Hydrochloride
MP BiomedicalsGuanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first…
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Urea, Ultra Pure
MP BiomedicalsUrea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle. Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured…
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Urea (Ultr Pure Grade)
G-BiosciencesFeatures Synonym: Carbamide, Carbonyldiamide Linear Formula: NH2CONH2 Molecular Weight: 60.06 CAS Number: 57-13-6
