Rockland Immunochemicals Inc Buffer Solutions
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1.0 M Tris HCl pH 8.5, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 8.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade (WFI) water. This product…
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BlockOut® (2X), 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncBlockOut® is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This product allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and…
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TMB ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.
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1X SDS-PAGE Destain Solution (Ready-to Use), 1L, Liquid
Rockland Immunochemicals Inc1X SDS-PAGE Destain Solution is ready-to-use as a working solution and requires no further dilution.
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1.0 M Tris HCl pH 9.0, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer…
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NPP (50X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 10mL, Liquid - clear to pale…
Rockland Immunochemicals IncMust be diluted
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Blocking Buffer
Rockland Immunochemicals IncBlockOut® allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye® 800 and IRDye® 700DX (Licor), Cy2™,…
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FREUND'S INCOMPLETE ADJUVANT
Rockland Immunochemicals IncFreund’s adjuvant may be used to form water-in-oil emulsions with antigens for the injection of immunogens for antibody production. Proper emulsion formation is essential and should be tested by observing a drop of the emulsion on the surface of water which should 'bead and not disperse. Antigens…
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20X SSPE Buffer (3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M…
Rockland Immunochemicals IncThis product is a 20X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4. Meticulously prepared…
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1X PBS Buffer with 1% Casein
Rockland Immunochemicals IncThis product is a ready-to-use 1X solution and no further dilution is required.
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Tris-Glycine
Rockland Immunochemicals IncTris-Glycine running buffer is suitable for laboratory involved in protein biochemistry. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X…
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NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale…
Rockland Immunochemicals IncNPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow, Expiration date is one (1) year from date of opening.
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10X Tris-Borate-EDTA (TBE) (0.9 M Tris-Borate 0.01 M EDTA pH 8.3)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Borate EDTA (TBE) consists of 0.9 M Tris-Borate, 0.01 M EDTA at a pH of 8.3. Meticulously prepared using ultra pure reagents…
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Express Transfer Buffer, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncExpress Transfer Buffer is a alternative to tris-glycine as a transfer buffer in the western blot. It may run at an elevated temperature but gives markedly lower background.
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Blocking Buffer for Immunohistochemistry, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis immunohistochemistry protein blocker is specifically designed for immunohistochemical staining where detection occurs using an immunoenzymatic reaction with substrate, i.e. peroxidase (HRP) with diaminobenzidine (DAB). This product specifically inhibits non-specific staining of IHC detection…
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10X BBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X BBS Fish Gel Concentrate consists of 1.0M boric acid, 1.5M sodium chloride and Fish Gelatin at pH 8.0. A proprietary…
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3.0 M Sodium Acetate pH 5.2 DEPC TREATED, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncSodium Ethanoate is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains 3.0 M Sodium Acetate at pH 5.2 meticulously prepared using ultra pure reagents dissolved in highly polished…
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Competent Cell Buffer (60 mM Calcium Chloride, 15% (v/v) Glycerol, 10 mM Tris…
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
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ALP Conjugate Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals IncDilute your primary or secondary alkaline phosphatase conjugated antibody or other protein or compound with ALP Conjugate Stabilizer to the desired 1X working concentration determined for your conjugate. ALP Conjugate Stabilizer may be used to dilute conjugates when determining titer. In one step…
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FREUND'S COMPLETE ADJUVANT
Rockland Immunochemicals IncFreund’s adjuvant may be used to form water-in-oil emulsions with antigens for the injection of immunogens for antibody production. Proper emulsion formation is essential and should be tested by observing a drop of the emulsion on the surface of water which should 'bead and not disperse. Antigens…
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UltraPure Sterile Water, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncUltra Sterile Water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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FETAL BOVINE SERUM Origin: United States triple 0.1 micron filtered
Rockland Immunochemicals IncFetal Bovine Sera sourced from the United States is collected exclusively from USDA licensed abattoirs from within the United States. Raw material is processed through 0.1 µm (100nm) filters in compliance with regulatory guidelines. Animals are inspected pre-mortem and post-mortem by USDA…
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10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED, 1L, Liquid (sterile…
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in…
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1.0 M Tris HCl pH 7.4, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 7.4. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or…
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Cell Lysis Buffer, 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThe buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.
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HRP Conjugate Stabilizer (Azide and Mercury Free), 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a ''ready-to-use'' 1X diluent for Horseradish Peroxidase (HRP) conjugated primary or secondary. This buffer contains buffered saline along with proprietary reagents to stabilize and preserve Horseradish Peroxidase (HRP) conjugated primary or secondary antibodies in solution. A…
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GET Buffer
Rockland Immunochemicals IncThis product is a 1X concentrated stock solution. No dilution is required. This buffer consists of 50 mM Glucose, 10 mM EDTA, and 25 mM TRIS Cl at a pH of 8.0. Meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade deionized water.
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0.5 M EDTA pH 8.0, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncEthylenediaminetetraacetic acid is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 0.5 M Trisodium EDTA at pH 8.0. Meticulously prepared using ultra pure reagents dissolved…
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BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk), 500g, Lyophilized
Rockland Immunochemicals IncUse BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a…
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1X RIPA Lysis Buffer 5-PACK (5 x 50 ml), 1Each, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein…
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PolyMount Mounting Media MaxTag™ Histo for IHC, 15mL, Liquid
Rockland Immunochemicals IncThis component is ready-to-use and requires no additional preparation.
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1X Lysis Buffer, 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncChill to 2 to 8 °C prior to use. Lysis Buffer is provided as a ready-to-use 1X solution containing 50 mM Tris Cl, 150 mM Sodium Chloride, 1 mM EDTA, 1% TRITON-X-100, pH 7.4. No preparation is required prior to use.