Rockland Immunochemicals Inc Buffer Solutions
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ProXtract B™ Extraction Buffer
Rockland Immunochemicals IncProXtract B™ Buffer is suitable for protein extraction and easily scalable. Specific conditions should be optimized by the end user. See protocol.
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10X BBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X BBS Fish Gel Concentrate consists of 1.0M boric acid, 1.5M sodium chloride and Fish Gelatin at pH 8.0. A proprietary…
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0.5 M Calcium Chloride, 500mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a 0.5M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains 0.5 M Calcium Chloride. Meticulously prepared using ultra pure reagents dissolved in highly polished…
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20X SSPE Buffer (3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M…
Rockland Immunochemicals IncThis product is a 20X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4. Meticulously prepared…
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Tris-Glycine
Rockland Immunochemicals IncTris-Glycine running buffer is suitable for laboratory involved in protein biochemistry. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X…
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Revitablot™ Western Blot Stripping Buffer
Rockland Immunochemicals IncRevitablot™ Western Blot Stripping Buffer is ready-to-use as a working 1X solution and requires no further dilution.
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Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or…
Rockland Immunochemicals IncPrepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B). Mix well. Protect from light. Larger or smaller volumes of the substrate can be prepared by…
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5.0 M Potassium Acetate (untitrated) DEPC TREATED
Rockland Immunochemicals IncThis product is a 5.0M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains untitrated 5.0 M Potassium Acetate. Meticulously prepared using ultra pure reagents dissolved in highly…
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10X SDS-PAGE Running Gel Buffer (0.25 M Tris, 1.92 M Glycine, 1.0% SDS pH 8.3),…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required.
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NPP BUFFER (1X WORKING BUFFER), 500mL, Liquid - clear
Rockland Immunochemicals IncUtilized as the diluent buffer in ELISA experiments
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10X Tris-Phosphate-EDTA (TPE) (0.9 M Tris-Phosphate 0.01 M EDTA pH 8.0), 1L,…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Phosphate EDTA (TPE) consists of 0.9 M Tris-Phosphate, 0.02 M EDTA at a pH of 8.0. Meticulously prepared using ultra…
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Competent Cell Buffer (60 mM Calcium Chloride, 15% (v/v) Glycerol, 10 mM Tris…
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
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BCIP/NBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale…
Rockland Immunochemicals IncBCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color…
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UltraPure Sterile Water (4 x 1 liter), 4L, Liquid (sterile filtered)
Rockland Immunochemicals IncSuitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals IncThis product is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to ELISA. After coating with antigen or antibody, wash the contents of the ELISA microwells and add a sufficient volume of ELISA Microwell Blocking Buffer with Stabilizer to each well of the microplate. …
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Express Transfer Buffer, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncExpress Transfer Buffer is a alternative to tris-glycine as a transfer buffer in the western blot. It may run at an elevated temperature but gives markedly lower background.
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TMB MEMBRANE PEROXIDASE SUBSTRATE. READY-TO-USE, 100mL, Liquid - clear
Rockland Immunochemicals IncTMB Membrane Peroxidase Substrate comes ready to use. No dilutions are required. TMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation…
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5.0 M Sodium Chloride DEPC TREATED, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a 5.0M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration.
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1.0 M Tris HCl pH 9.0, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer…
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FETAL BOVINE SERUM, Certified, Heat Inactivated Origin: United States triple 0.1…
Rockland Immunochemicals IncHeat inactivated fetal bovine serum is for in vitro diagnostic use, not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. Certified Fetal Bovine Sera meets USDA requirements for abattoir-sourced animals, traceability and country of…
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Blocking Buffer for Fluorescent Western Blotting THIMEROSAL FREE 10-PACK (10 x…
Rockland Immunochemicals IncOptimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance. This buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary…
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1.0 M Tris HCl pH 7.4, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 7.4. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or…
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Borate Saline Buffered (BSB) (100 mM Boric Acid, 25 mM Sodium Borate, 75 mM…
Rockland Immunochemicals IncBorate Saline Buffered (BSB) is commonly used in coating procedures for ELISA. BBS can also be used as a component in blocking solutions for Western blotting. The buffer can be used as a diluent in biological samples and for various applications within biology and biochemistry to maintain the pH.
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Ponceau S solution, 500mL, Liquid
Rockland Immunochemicals IncStore at room temperature. The product is at working concentration, no further dilution is necessary. The limit of detection for this stain is 200 nanograms of protein after separation by electrophoresis in polyacrylamide gels and transferred to nitrocellulose or PVDF membranes. Ponceau S is not…
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Cell Lysis Buffer, 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThe buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.
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10% (w/v) Sodium Dodecyl Sulfate (SDS), 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly…
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1X RIPA Lysis Buffer 5-PACK (5 x 50 ml), 1Each, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein…
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Blocking Buffer (10X) for Fluorescent Western Blotting THIMEROSAL FREE, 50mL,…
Rockland Immunochemicals IncThis buffer is a 10X concentration. Optimum performance is achieved using this product diluted 1:10 in deionized water. This buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6.
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Blocking Buffer for Fluorescent Western Blotting, 125mL, Liquid (sterile…
Rockland Immunochemicals IncFluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications.…
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ABTS ELISA PEROXIDASE SUBSTRATE, 100mL, Liquid - clear to very pale green
Rockland Immunochemicals IncABTS ELISA PEROXIDASE SUBSTRATE is a ready to use substrate - No dilution required. Read absorbance of blue green color at 405 nm. The color formation as a function of time can be recorded at intervals or the reaction may be stopped by addition of acid after 30 mins. A 0.625 Mol L-1 Oxalic Acid…
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1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED, 1L, Liquid …
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1.0 mM EDTA at a pH of 7.8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.
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BLOTTO B PRE-MIXED, 500mL, Lyophilized
Rockland Immunochemicals IncUse BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a…