PIPES is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work.

MOPSO sodium salt is a zwitterionic aminosulfonate buffer that is very similar in structure to MOPS (3-morpholinopropanesulfonic acid), differing by the presence of a hydroxyl group on C-2 of the propane moiety.

Tris is an excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per…

Tris-HCl is excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per…

HEPPS is used as a separator in ultrathin isoelectric focusing gels; enhances the resolution of phosphoglucomutase. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration).

Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested. …