Buffers

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  • PIPES Buffer (0.5 M, pH 8.0)

    Boston BioProducts

    Components: PIPES(500 mM), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: 2-8°C

  • MOPS Buffer (0.2 M, pH 5.5)

    Boston BioProducts

    Components: MOPS(~4.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.5 ± 0.15 Storage: 2-8°C

  • Components: Tris Base / Tris-HCl(200 mM), EDTA Disodium Dihydrate(20 mM) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle and/or filtered through 0.22-micron filter. pH: 8.0 ± 0.15 (1X) Storage: Room Temp.

  • Components: Sodium Phosphate Dibasic(10 mM), Potassium Phosphate Monobasic(2 mM), Potassium Chloride(2.7 mM), Sodium Chloride(137 mM), Triton X-100(1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage:…

  • DIPSO Buffer (0.2 M, pH 7.5)

    Boston BioProducts

    Components: DIPSO(200 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(200 mM), Potassium Phosphate Monobasic(40 mM), Potassium Chloride(54 mM), Sodium Chloride(2.75 M), Tween-20(1%), Kathon(0.02%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid…

  • PIPES Buffer (1 M, pH 6.8)

    Boston BioProducts

    Components: PIPES(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.8 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(10 mM), Potassium Phosphate Monobasic(2 mM), Potassium Chloride(2.7 mM), Sodium Chloride(137 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

  • Tris-HCl Buffer (1 M, pH 9.5)

    Boston BioProducts

    Components: Tris Base / Tris-HCl(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 9.5 ± 0.2 Storage: 2-8°C

  • Components: HEPES(50 mM), Potassium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: 2-8°C

  • Components: Tris Base / Tris-HCl(250 mM), Sodium Chloride(1.37 M), Potassium Chloride(27 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: HEPES(1 M), Potassium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: 2-8°C

  • Components: Tris Base / Tris-HCl(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.2 ± 0.2 Storage: 2-8°C

  • Borate Buffer (0.5 M, pH 9.5)

    Boston BioProducts

    Components: Boric Acid(0.5 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 9.5 ± 0.15 Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(1 M), Sodium Chloride(3 M), Tween-20(2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.

  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(200 mM), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.5 ± 0.15 Storage: Room Temp.

  • Components: Potassium Phosphate Monobasic(20 mM), Potassium Phosphate Dibasic(100 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage:…

  • Maleate Buffer (0.2 M, pH 6.0)

    Boston BioProducts

    Components: Maleate(0.2 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.0 ± 0.15 Storage: Room Temp.

  • Components: Sodium Phosphate Dibasic(200 mM), Potassium Phosphate Monobasic(40 mM), Potassium Chloride(54 mM), Sodium Chloride(2.75 M), Tween-20(2%), Sodium Azide(0.02%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on…

  • Components: Citric Acid / Sodium Phosphate Dibasic(100 mM / 200 mM mixture), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.5 ± 0.15 Storage: 2-8°C

  • Components: Tris Base / Tris-HCl(1 M), Sodium Chloride(3 M), Tween-20(2%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • MOPS Buffer (0.2 M, pH 7.5)

    Boston BioProducts

    Components: MOPS(~4.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: 2-8°C

  • EDTA (14%, pH 7.4)

    Boston BioProducts

    Components: EDTA(14%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 Storage: Room Temp.

  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(200 mM) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(0.2 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(200 mM), EDTA(20 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle and/or filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • MES Buffer (1 M, pH 9.0)

    Boston BioProducts

    Components: MES Monohydrate(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 9.0 ± 0.15 Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(250 mM), Sodium Chloride(1.37 M), Potassium Chloride(27 mM), Tween-20(1%), Sodium Azide(0.02%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.6 ± 0.15 (1X) …

  • Components: Sodium Phosphate Dibasic(100 mM), Potassium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Tween-20(1%), Kathon(0.02%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid…

  • Components: Potassium Phosphate Monobasic / Potassium Phosphate Dibasic(200 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(500 mM), Sodium Chloride(2.75 M), Potassium Chloride(54 mM), Tween-20(2%), Sodium Azide(0.02%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.6 ± 0.15 (1X) …

  • RIPA Buffer (2X, pH 8.0)

    Boston BioProducts

    Components: Sodium Dodecyl Sulfate (SDS)(0.2%), Tris Base / Tris-HCl(100 mM), Sodium Chloride(300 mM), Nonidet P-40 Substitute(2%), Sodium Deoxycholate(1%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 (1X) …

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