Cayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO. The reaction between hydrogen peroxide and…

Water used for the preparation of EIA reagents and buffers.

Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative…

ALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and…

Glycogen is the principal storage form of glucose in animal and human cells. It is made primarily by the liver and muscles, but it can also be made by glycogenesis within the brain and stomach. Cayman’s Glycogen Assay provides a simple, reproducible, and sensitive tool for assaying glycogen…

Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that…

11-KT is a second key androgenic steroid found in fish. It occurs in males together with testosterone in amounts which vary from less than 1 ng/ml to as much as 50-100 ng/ml, depending on the species and the stage of the reproductive cycle.

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, beta (NR1B2), a ligand-dependent transcription factor commonly referred to as RARB or RARβ. INDIGO’s reporter cells…

Features Allows insulin measurement even in hemolyzed samples Contains insulinase inhibitors to protect sample integrity during collection The main analytical pitfalls in insulin measurement in serum are related to (a) hemolysis, (b) circulating anti-insulin autoantibodies, and, for…

Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated…

Complex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to fumarate, and in the process reduces ubiquinone (Q) to ubiquinol (QH2). Ultimately,…

Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration, and as a major source of reactive oxygen species III. It accepts electrons from complexes I and II in the form…

4-(hydroxymercuri)Benzoic Acid(PHMB) is used to inhibit cysteine-protease and acts as a reversible thiol-specific blocker. While blocking the acylated ghrelin degradation, it allows the AcetylCholine Esterase AChE™ used in SPI-Bio EIA kits to work normally, in contrast to serine-protease inhibitors.

Cayman’s PC Assay provides a specific, sensitive, and convenient method for quantifying PC in plasma or serum. In this assay, PC-Specific PLD hydrolyzes PC to choline and phosphatidic acid. The newly formed choline is then used to generate hydrogen peroxide in a reaction catalyzed by choline…

This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human vitamin D receptor (NR1I1), a ligand-dependent transcription factor commonly referred to as VDR. INDIGO’s reporter cells include the luciferase reporter…

NADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH used in a…

Urinary creatinine levels are commonly used as an index of standardization for a variety of other tests. Measurement of creatinine clearance is also useful in detecting renal disease and estimating the extent of impairment of renal function. The Cayman Chemical Creatinine (urinary) Colorimetric…

Myeloperoxidase (MPO), released from neutrophils to degrade invading pathogens, provides one of the earliest lines of defense in innate immunity. It catalyzes the hydrogen peroxide-mediated oxidation of halide ions to products such as hypochlorous acid (HOCl). These reactive products participate in…

Pyruvate (pyruvic acid) is a key intermediate in cellular metabolic pathways and is derived primarily from glucose via glycolysis. Abnormal blood pyruvate levels are reported in a number of disorders including shock, liver disease, congestive heart failure, diabetes mellitus, thiamine deficiency,…

Interleukin-6 (IL-6) is produced by monocytes, macrophages, and T cells and is invovled in many defense mechanisms including the immune response, hematopoiesis, bone metabolism, and acute phase reactions. Cayman’s IL-6 (mouse) ELISA Kit is an immunometric (i.e., sandwich) ELISA that permits…

Prolactin is a polypeptide hormone synthesized and secreted by the anterior pituitary gland, placenta, decidua, and by various immune system cells, such as T cells, B cells, and NK cells. It has many functions, including stimulating mammary glands to produce milk (lactation), stimulating surfactant…

The oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from healthy oxidative phosphorylation to aerobic glycolysis in cancer cells. Oxygen…

For the measurement of acylated ghrelin.

CGRP is a 37 amino acid peptide synthesized in the central and peripheral nervous system from a calcitonin/CGRP gene complex. Two isoforms have been described which differ by three amino acids and display similar biological activities: CGRP-α, which is produced by alternative splicing of a…

Immunization of mice with citrullinated human fibrinogen, especially mice expressing the human HLA-DR4 transgene, induces an arthritic response driven by the production of antibodies that recognize citrullinated epitopes. The polyclonal antibody response produces antibodies reactive with both…

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human liver X receptor beta (NR1H2), a ligand-dependent transcription factor commonly referred to as LXRβ. INDIGO’s reporter cells include the…

Cayman’s MTT Proliferation Assay provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation. In this assay, MTT is taken up by cells and…

INDIGO’s panel of TR reporter assays utilizes non-human mammalian cells engineered to express human thyroid hormone receptors alpha (NR1A1) and beta (NR1A2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. INDIGO’s TR reporter cells include the…

Substance P is a bioactive 11-amino acid peptide first isolated in 1931 from brain and intestine. The peptide is involved in many physiological processes including pain modulation, smooth muscle contraction, blood pressure control, kidney function, and water homeostasis. Substance P is widely…

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman’s Direct Fluorescent Screening Assay Kits provide a convenient…

This assay has been developed for researchers that do not have experience performing ELISAs. It can be used as a practice tool allowing you to become comfortable with running Cayman ELISAs. This kit contains enough reagents to run at least four complete standard curves.

Luteinizing hormone (LH) is a glycoprotein produced by the pituitary gland and consists of two subunits with a total molecular mass of approximately 30 kDa. The α-subunit is identical to the α-subunit of other pituitary hormones, such follicle stimulating hormone (FSH), thyroid…