The Large DNA Fragment Extraction Kits are designed to recover large DNA fragements (> 8Kb) from an agarose gel. The Small DNA Fragment Extraction Kits are design to recover or concentrate small DNA fragments (50-200bp) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. The entire procedure can be completed in 45 minutes (Large) or 20 minutes (Small) and the DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling, and Ligation.
Key Features:
- SAMPLE SIZE: Large - up to 300mg of agarose gel; Small - up to 300mg of agarose gel or up to 100μl of PCR products
- FORMAT: Spin Column
- OPERATION: Centrifuge / Vacuum Manifold
- EXPECTANT YIELD: Large - Up to 85% / Small - Up to 95%
- OPERATION TIME: Large - 45 minutes or less / Small - 20 minutes or less
- APPLICATIONS: PCR, Fluorescent or Radioactive Sequencing, Restrictive Digestion, DNA Labeling, Ligation
Kit Contents (Small Fragment)
| Component | C997K54 | C997K55 | C997K56 |
| SD COLUMNS | 4 | 100 | 300 |
| 2ML COLLECTION TUBES | 4 | 100 | 300 |
| SD BUFFER | 3 mL | 80 mL | 240 mL |
| W1 BUFFER | 2 mL | 45 mL | 130 mL |
| WASH BUFFER (add Ethanol) | 1 mL (4 mL) | 25 mL (100 mL) | 50 mL + 100 mL (200 mL)(100 mL) |
| ELUTION BUFFER | 1 mL | 6 mL | 30 mL |
Kit Contents (Large Fragment Gel Extractions Only)
| Component | C997K57 | C997K58 | C997K59 |
| LD COLUMNS | 4 | 100 | 300 |
| LD BUFFER | 100 ul | 3 mL | 25 mL |
| WASH BUFFER (add Ethanol) | 1 mL (4 mL) | 25 mL (100 mL) | 50 mL + 25 mL (200 mL)(100 mL) |
| RESUSPENSION BUFFER | 1 mL | 6 mL | 30 mL |