Collagen, Type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered

Synonyms: Collagen from rat tail

CAS Number: 9007-34-5

MDL No.: MFCD00130825

Storage: 2-8C

EC Number: 232-697-4

UNSPSC Code: 12352200

Application: Used as a coating material to support adherent cells growth and differentiation.

Preparation Note: Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2.SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.

Preparation Note: Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.

Other Notes: Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.