HPG (p-hydroxyphenylglyoxal) reacts to specifically modify arginine residues under mild conditions to yield spectrophotometrically measurable signal for amino acid detection.
Features of HPG:
• Arginine-specific—reacts specifically with arginine residues under mild conditions (pH 7 to 9, 25°C)
• Quantitative—reaction follows Beer's Law at 5 to 50 µM and can be monitored at 340nm (pH 9)
• Superior to alternatives—more resistant to oxidation than p-nitrophenylglyoxal and more water-soluble than phenylglyoxal
Features
- Alternative names: HPG monohydrate
- Chemical name: (p-Hydroxyphenyl)glyoxal; (4-Hydroxyphenyl)(oxo)acetaldehyde
- Reactive toward: Arginine (Arg, R)
- Chemical formula: C8H6O3
- CAS number: 197447-05-5; 24645-80-5
- Molecular weight: 150.13 (supplied as monohydrate: 168.15)
- Form: Off-white crystalline powder