MycoZap Mycoplasma Elimination Reagent removes detectable mycoplasma contamination in as little as four days and has been optimized to clear Mycoplasma with minimal toxic effects to the contaminated cells. The reagent employs a combination of antibiotic and antimetabolic agents and offers reliable elimination of Mycoplasma to a standard that cannot be achieved using antibiotics alone. The MycoZap Elimination Reagent can be used to remove a large range of Mollicutes, including Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma, from cell cultures.