Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking) is a hot-start EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ features a unique combination of a master mix containing a low concentration of blue dye, plus a DNA template buffer containing a higher concentration of blue dye. This 2-color tracking system allows you to see at a glance whether you forgot to add master mix or template to any of your reactions, so you can catch pipetting mistakes and avoid wasting time, reagents, and your precious DNA samples. The master mix features EvaGreen® Dye which is suitable for HRM analysis and is safer, more environmentally friendly, and more sensitive than SYBR® Green. The kit also features Cheetah™ Hotstart Taq for fast cycling protocols. This master mix is available withh no ROX or with separate ROX reference dye for use with any PCR instrument.