Biotium
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Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking) is a hot-start EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ features a unique combination of a master mix containing a low concentration of blue dye, plus a DNA template…
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Forget-Me-Not™ EvaGreen® qPCR Master Mix is a high performance, hot-start EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ master mix has a light blue master mix color which helps prevent pipetting mistakes, saving time and reagents.…
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AM1-43
BiotiumAM1-43 is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly similarly to SynaptoGreen C4 (catalog # 70020), but with the additional property of being fixable.
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Propidium Iodide
BiotiumPropidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.
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The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.
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GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed® is far more sensitive than EB without requiring a…
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Mini Cell Scrapers
BiotiumMini Cell Scrapers for harvesting cells or cell lysates from 96-, 48- and 24-well plates. Features: Size: 0.5 cm (3/16″) in width and 6 cm (2 3/8″) in length. Packaged in 20 sterile packs of 10 scrapers per pack. Polyethylene, disposable & sterile.
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This product is prepared by labeling highly cross-adsorbed donkey anti-human IgG (H+L) with a selection of our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, mouse, rabbit, sheep and…
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ARP
BiotiumARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated…
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Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Biotium’s Steady-Luc™ HTS assay system is a homogeneous firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of…
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Acridine Orange
BiotiumAcridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm).The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.
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AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm). The detection range of the assay is 0.1-15 ug/mL protein.
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6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on agarose or polyacrylamide gels, with two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. To use, mix 1 uL of 6X DNA Loading Buffer for every 5 uL DNA sample…
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The Apoptosis & Necrosis Quantification Kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.
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Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This kit performs at least as well as other commercial resazurin-based cell proliferation assay kits such as alamarBlue® or CellTiter-Blue®.
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DAPI
BiotiumDAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement.
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EvaGreen® Dye, 20X in Water
BiotiumEvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.
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SynaptoRed™ C2
BiotiumA widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™, synapses or neuromuscular junctions can be imaged independently in two colors.…
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Goat Anti-Mouse IgG (H+L)
BiotiumThis product is prepared by labeling high quality goat anti-mouse IgG (H+L) with a selection of our bright and photostable CF® dyes and other labels.
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Phalloidin Conjugates
BiotiumPhalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF® dye phalloidin conjugates consist of the mushroom toxin phalloidin labeled with our superior bright and photostable CF® fluorescent dyes.
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10X Fish Gelatin Blocking Agent
Biotium10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum…
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GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen® is far more sensitive than SYBR Safe. We continue to offer GelGreen® 10,000X solution in DMSO for established users who…
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Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and…
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ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and are used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments.…
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PMA Dye, 20 mM in H2O
BiotiumPMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive DNA-binding dye that is useful for determination of microbial viability by qPCR.
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The CF® dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.
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The Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells provides green/red fluorescent staining of live and dead cells, respectively. The assay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells.
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The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.01-10 ng/uL.
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NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.
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Fast Evagreen® qPCR Master Mix
BiotiumFast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial…
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Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF® dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody…
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XTT Cell Viability Assay Kit
BiotiumXTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using standard photometric microplate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and to screen cytotoxic agents.