This product is similar to biotin-11-dUTP and biotin-16-dUTP except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.    Lyophilized solid suitable for long term storage; contains lyophilized TE…

AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in…

GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed® is far more sensitive than EB without requiring a…

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry.

Annexin V conjugated to R-phycoerythrin (R-PE or PE) can be used to detect apoptotic cells by flow cytometry. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the…

AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in…

NBD-PE (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, triethylammonium salt) in combination with Rhodamine-DHPE (#60026) or Texas Red®-DHPE (#60027) has been used to study membrane fusion via fluorescence resonance energy transfer (FRET). NBD-PE has also…

The AccuGreen High Sensitivity dsDNA Quantitation Kit for Handheld Fluorometers is designed for DNA Quantitation using a small fluorometer such as the Qubit. The AccuGreen kit contains everything you need for sensitive, accurate dsDNA quantitation on the Qubit fluorometer.

NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.

6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on agarose or polyacrylamide gels, with two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. To use, mix 1 uL of 6X DNA Loading Buffer for every 5 uL DNA sample…

CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells.

CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues.

Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading.    K_d: 250 nM   &lamba;_Ex/&lamba;_Em (no Ca 2 ) = 349/482 nm   &lamba;_Ex/&lamba;_Em (high Ca 2 ) = 331/398 nm…

PMA-PCR kits are designed for selective detection of viable bacteria from a specific strain using PMA dye and real-time PCR. The kits contain PMA dye, Forget-Me-Not™ qPCR Master Mix, and PCR primers for detection of selected strains of bacteria that are of widespread interest to food safety, public…

Polyclonal rabbit anti-V5 tag (GKPIPNPLLGLDST) is designed to detect V5 tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The antibody recognizes the epitope tag fused to either the amino- or…

Dilinoleyl DiO (1,1'-dilinoleyl-3,3'-oxacarbocynanine perchlorate), also known as FAST DiO, has identical absorption and emission spectra as DiO. However, due to its unsaturated hydrocarbon chains, dilinoleyl DiO has a much faster lateral diffusion rate in cell membranes than DiO.    …

Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange fluorescent CF®543 dye has…

This product is prepared by labeling an affinity-purified chicken anti-mouse IgG (H+L) with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent…

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an…

Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This kit performs at least as well as other commercial resazurin-based cell proliferation assay kits such as alamarBlue® or CellTiter-Blue®.

GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed® is far more sensitive than EB without requiring a…

This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF®568 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF®568…

Our CF® dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more…

Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial…

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF®647 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red…

Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq. The master mix is offered in…

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility…

Fluorescent conjugates of Alpha-Bungarotoxin bind to the nicotinic acetylcholine receptor with high affinity, and thus can be used to image the distribution of the receptor at the neuromuscular junctions. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness,…

ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay procedure involves a single addition of ATP-Glo™ Detection Cocktail directly to cells cultured in a serum-supplemented medium.

The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.01-10 ng/uL.

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and…

This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with CF®405L dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green…