Benedict's reagents comprise of copper sulphate, sodium carbonate which functions as a mild alkali, and sodium citrate. It is quite stable. On boiling this reagent with glucose, it gives a white precipitate of cuprous thiocyanate. It is primarily used for the estimation of glucose in urine.
MDL Number: MFCD00146068
Application: Benedict's reagent may be used during TLC analysis of mesocotyl methanol extracts during a study to evaluate sorghum resistance associated with accumulation of undetected toxic host metabolites. It may also be also be used to synthesize an SEM image of the cubic shaped Cu2O with smooth surface.
Other Notes: Reagent used for the detection of reducing sugars and other reducing substances; detection limit: 0.01 % glucose in water
Impurities: cupric sulfate