Synonyms: 3,3'-Sulfinylbis(N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)propanamide); Mass spectrometry-cleavable crosslinker for studying protein-protein interations
Molecular Formula: C18H22N4O7S
Molecular Weight: 438.45
Linear Structural Formula: C18H22N4O7S
Purity: >=95%
Storage: 2-8C
Application: BMSO (bismaleimide sulfoxide) crosslinker is a homobifunctional, cysteine residue-targeting, sulfoxide-containing crosslinker for analysis of protein-protein interactions (PPIs) through crosslinking mass spectrometry (XL-MS). BMSO possesses two maleimide reactive groups for targeting Cys, a 24.2 Å spacer arm, and two symmetrical C-S cleavable bonds adjacent to the central sulfoxide. While similar reagents react with lysine (Lys) and acidic residues, targeting Cys provides further opportunity to characterize protein interaction surfaces. Additionally, the post-cleavage spacer yields tagged peptides for unambiguous identification by collision-induced dissociation in tandem MS.BMSO crosslinker provides complementary data to amine-reactive and acidic residue-targeting reagents and will find wide utility in the elucidation of PPIs, study of proteins that function as complexes, quantification of structural dynamics, and the quest for targeting "undruggable" protein targets.
Legal Information: Subject to US provisional patent application #62/845,240 of the Regents of the University of California
Other Notes: Technology Spotlight: Cross-Linkers for Elucidation of Protein-Protein InteractionsDevelopment of a Novel Sulfoxide-Containing MS-Cleavable Homobifunctional Cysteine-Reactive Cross-Linker for Studying Protein-Protein InteractionsStructural dynamics of the human COP9 signalosome revealed by cross-linking mass spectrometry and integrative modeling