Source: Active recombinant enzyme expressed in E. coli
A soluble enzyme induced by LPS and cytokines that is responsible for the biosynthesis of NO from L-arginine; one unit of enzyme produces 1 µmol of NO per minute at 37°C in 50 mM HEPES, pH 7.4, containing 1 mM arginine, 1 mM magnesium acetate, 5 µM oxyhemoglobin, 0.1 mM NADPH, 12 µM tetrahydrobiopterin, and 170 µM DTT