RocketScriptTM Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistnace to heat (42 ~ 70?) allows effective synthesis of complex RNA structures with full-length cDNA. Features and Benefits
Thermostablity
Performance at comprehensive temperature conditions from 42 to 70 ? for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions
User-friendly
Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.
Application
- Gene synthesis
- First-strand synthesis of cDNA from RNA molecules (reverse transcription)
- RT-PCR
- Random priming reactions
- Library construction
- Probe labeling
- mRNA 5'-end mapping by primer extension analysis
- Real time PCR