UNSPSC Code: 41106000
RIDADR: NONH for all modes of transport
The PCR DIG Labeling Mix is specifically designed for the sensitive detection of PCR products and for the sensitive analysis of PCR reactions. When using higher concentrations of Digoxigenin-dUTP what is supplied with the PCR DIG Labeling Mix, the yield of the PCR product may be reduced, but however, the label intensity and the molecular weight of the PCR product is increased. The PCR DIG Labeling Mix can also be used for the synthesis of hybridization probes. However, for the production of highly sensitive probes, for example, necessary when detecting single-copy genes on genomic blots, we recommend a PCR nucleotide mix with an increased concentration of Digoxigenin-dUTP (e.g., PCR DIG Probe Synthesis Kit).
This nucleotide mixture can be added directly to polymerase chain reactions and the digoxigenin-labeled nucleotide will be incorporated into the PCR product. Taq DNA polymerase, as well as Tth DNA polymerase, can be used for the synthesis of digoxigenin-labeled PCR products. The PCR DIG Labeling Mix can replace the unlabeled nucleotide mix in PCR. 10μl of the PCR DIG Labeling Mix is used in a standard 100μl PCR assay.
For life science research only. Not for use in diagnostic procedures.
The PCR DIG Labeling Mix is function tested in PCR. Amplification products are assayed by dot blot and in hybridization experiments. DNases and RNases are not detectable according to the current Quality Control procedures.
Solution, 10x concentrated: PCR DIG labeling mix is a mixture of the sodium salts of dATP, dCTP, dGTP, dTTP and digoxigenin-11-dUTP, lithium salt. The solution contains 2 mM dATP, dCTP, dGTP each, 1.9 mM dTTP, 0.1 mM digoxigenin-11-dUTP (DIG-11-dUTP) in 2x 250 μl water; pH 7.0.