ROCHE Expand™ High FidelityPLUS PCR System

Synonyms: high fidelity pcr plus system, expand

Storage: −20°C

UNSPSC Code: 12352200

Components: Expand High Fidelity^PLUS Enzyme Blend 5 U/μl; Expand High Fidelity^PLUS Reaction Buffer, with 7.5 mM MgCl₂ 5x concentrated; Expand High Fidelity^PLUS Reaction Buffer, without MgCl₂ 5x concentrated; MgCl₂ Stock Solution 25 mM

RIDADR: NONH for all modes of transport

Features and Benefits:
• Safeguard against cross-contamination.
This is the choice for large numbers of amplifications.
• Obtain sixfold higher fidelity and higher yields,
compared to Taq DNA Polymerase.
• Efficiently label DNA.
Use this kit to label DNA with radioactive or nonradioactive nucleotides.

Application:
The Expand^™ High Fidelity^PLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:
• PCR
• RT-PCR
• Primer extension
• Carryover prevention

General description:
The Expand High Fidelity^PLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High Fidelity^PLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High Fidelity^PLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Quality:
Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Legal Information:
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.