Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) - Compatible with: ABI-R- 7000, 7300, 7700, 7900, StepOnePlus-R- StepOne, Eppendorf-R- Realplex 4: This series of kit is designed for quantitative real-time analysis of DNA samples by measuring the increase in fluorescence caused by the binding of Green-2-Go dye to double-stranded (ds) DNA.
It is available in a ready-to-use format, compact with a wide range of real-time cyclers. Green-2-Go qPCR Mastermix is a convenient premix of all the components, 2X mix of dNTPs, Hotstart Taq polymerase, MgCl2, fluorescent detection dye, reference dye, and proprietary buffer components.
The components of Green-2-Go qPCR Mastermix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our mastermix significantly reduces nonspecific PCR amplification observed with regular Taq polymerase.
Due to variations in qPCR instruments, we offer different Green-2-Go qPCR Mastermix formulations optimized for different machines. Please use the following table as a guideline for the selection of qPCR Mastermix appropriate for your particular instrument model.