Components
Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.
General description
2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis. The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination. The hot start properties allow reaction setup at ambient temperature. With this robust reagent, any PCR protocol optimization is minimized